Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

Enzymes Technology - Enzymic Hydrolysis (Apr/01/2011 )

Dear all.
I have enzyme with declared activity of 7U/mg. I need to use in enzymic hydrolysis to my substrate containing 1.3% protein. How much enzyme should be added in term of E/S ratio.


need more information:

what is unit definition?

is substrate protein or do you need to add enzyme until the resulting solution is 1.3% protein?