how to make a recombinant enzyme refolded - (Mar/15/2012 )
Hi, I am interested in an enzyme in a G(+) bacteria. It natually sits on cell membrane.
I have cloned it in E.coli. But the recombinant protein didn't have enzyme activity (I checked it by loading E coli on zymogram).
My question is: how to make a recombinant enzyme have activity? Does the procedure differs a lot for different enzymes, or there is a universal procedure?
Thanks very much! Look forward to hear from you!
you may find out an in vitro protocol for reconstitution (f.i. Novogene)
or try coexpression with a chaperon/HSP
Thanks! I am working on it.