Inclusion Body purification - (Feb/23/2010 )
I have two recombinant proteins that are expressed as inclusion bodies and when I try to purify these inclusion bodies I obtain many contaminants. I have been using a buffer composed by sodium phosphate 20 mM, NaCl 500mM and Imidazole 10 mM, followed by lisozyme (1mg/mL) and sonication, in bacterial lysis process. Then I use the same lysis buffer + Urea 8M to denaturate my protein. Does anybody know how can I obatin inclusion bodies with high purity?
Thanks very much!
KAUSHIK THAKKAR on Feb 24 2010, 06:12 AM said:
Hope this helps.
Thank you very much. I will try this protocol.