Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

Detection of SMALL Peptide (5 amino acids in size) - (Jun/21/2015 )



I'm still googling and searching through PubMed....

Does anyone know a method by which I can detect peptide fragment which are about 5 amino acids in length?

I guess Western Blot won't work for proteins that size?


Best regards and thanks in advance,



you can use tris-tricine sds-page (and immunoblot if you have an antibody against the peptide).


you can also separate the peptide and identify with hplc (especially if connected to a mass spectrometer).