Immunoprecipitation- Incubation time with beads - (May/17/2013 )
In all the protocols I see it is written to incubate the Lysate solution with the antibody overnight and then add the beads next day for an hour or so. I made a mistake and add the beads last night. They are incubated with AB and sample all over the night. Is it going to be a problem? Am I going to have a huge background? should I increase the wash? I pre-cleared the sample with Rabbit serum and beads before.
Please help me
You may have background but if you have pre-cleared the lysate this shouldn't be too much of a problem. The only way to know is to test it...