Whole brain homogenziation for coIP - (Feb/07/2018 )

Hi,

I want to homogenize a whole brain from a P5 or P50 mouse. Currently, I am using a dounce homogenizer to homogenize the brain in Lysis buffer. 

However, I am getting a low yield of proteins from the brain. I sometimes see a large chunk after the lysis. I am unclear if that is causing a low yield of protein and how can I homogenize a whole brain without leaving any large chunk of tissue or membrane.

After protein extraction, I follow it up with coIP.

Try Potter-Elvehjem  or Tenbroek grinders first for whole tissue. You can follow this with a Dounce if needed.