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421. false positive in colony screening PCR? - (reply: 8)
423. Protein expression in E.coli DH5alpha? - (reply: 1)
425. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
427. large plasmid extraction? - how? (reply: 5)
429. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
431. no GFP in cells - (reply: 7)
433. Random Amplification + TA cloning = HATE - (reply: 2)
435. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
437. Site-Directed Mutagenesis Kit - (reply: 1)
439. no plasmid after extraction - (reply: 2)
441. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
443. Blunt end cloning - vector and insert preparation (reply: 9)
445. P/C/I extraction of plasmid after digestion - (reply: 1)
447. Recommendations for a good site-directed mutagenesis kit - (reply: 3)
449. Gel extraction problem ~HELP~ - (reply: 3)
423. Protein expression in E.coli DH5alpha? - (reply: 1)
425. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
427. large plasmid extraction? - how? (reply: 5)
429. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
431. no GFP in cells - (reply: 7)
433. Random Amplification + TA cloning = HATE - (reply: 2)
435. Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
437. Site-Directed Mutagenesis Kit - (reply: 1)
439. no plasmid after extraction - (reply: 2)
441. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
443. Blunt end cloning - vector and insert preparation (reply: 9)
445. P/C/I extraction of plasmid after digestion - (reply: 1)
447. Recommendations for a good site-directed mutagenesis kit - (reply: 3)
449. Gel extraction problem ~HELP~ - (reply: 3)