Protocol Online logo
Top : Forum Archives: : Molecular Cloning

Electroporation with Pseudomonas strains - (Nov/20/2007 )

So guys
Who has tried electroporation with Pseudomonas strains? I am going to do electroporatically transform them with a shuttle/expression vector. Any body has a nice and easy protocol to prepare such electrocompetent cells. and How should I do it?


I workes in a lab with Pseudos before and they said, you can't make electrocompetent cells...they always did tri parental mating...