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Forum Archives: :
241. digestion of pcr - (reply: 2)
243. Plasmid lost during culture - (reply: 19)
245. double digestion and ligation - (reply: 3)
247. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
249. Ligation: time and temperature - (reply: 8)
251. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
253. Help restriction analysis of plasmid - (reply: 11)
255. Can I store plasmid in 4C refrigerator? - (reply: 11)
257. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
259. Trying to cleave out unwanted nucleotide sequence in an insert. - (reply: 1)
261. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
263. Many problems in genomic library construction - try to construction genomic library (reply: 3)
265. ligation - very small insert - problems during ligation (reply: 11)
267. Multimerization after restriction digestion - (reply: 9)
269. pcr template amount - (reply: 2)
243. Plasmid lost during culture - (reply: 19)
245. double digestion and ligation - (reply: 3)
247. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
249. Ligation: time and temperature - (reply: 8)
251. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
253. Help restriction analysis of plasmid - (reply: 11)
255. Can I store plasmid in 4C refrigerator? - (reply: 11)
257. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
259. Trying to cleave out unwanted nucleotide sequence in an insert. - (reply: 1)
261. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
263. Many problems in genomic library construction - try to construction genomic library (reply: 3)
265. ligation - very small insert - problems during ligation (reply: 11)
267. Multimerization after restriction digestion - (reply: 9)
269. pcr template amount - (reply: 2)