661. pBJ5 sequence - (reply: 1)
663. How to get rid of satellite colonies? - (reply: 17)
665. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
667. does the high pH of milli Q water will affect my ligation? - (reply: 8)
669. Weird thing: Cml resistant S. aureus strain (RN4220) - (reply: 1)
671. Issues with pcDNA3.1Directional cloning - (reply: 2)
673. Please save me ! - pAdeasy adenovirus vector system (reply: 1)
675. Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
677. E.coli + pCAMBIA1302 - everything is more complicated than expected... (reply: 4)
679. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
681. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
683. problems in ligation/transformation - (reply: 12)
685. wrong sequencing of T clones - (reply: 3)
687. Cloning with linkers? - (reply: 5)
689. Blunt end cloning PCR product - (reply: 6)

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