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1051. can colony-PCR screen for big inserts? - (reply: 3)
1053. Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
1055. Is dephosphate needed? - (reply: 6)
1057. Restriction digestion by SpeI and SphI - (reply: 2)
1059. High transformation background in negative control - (reply: 8)
1061. Amplifying a 6.5 kb cDNA - (reply: 3)
1063. pBluescript cloning of PCR products - (reply: 1)
1065. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
1067. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
1069. Reason for white colonies on outer rim of plates - (reply: 9)
1071. SEAP Assay for promoter activity - (reply: 2)
1073. From yeast to mamall cells - principles and methods online? (reply: 7)
1075. E. coli strains ER2566 - characteristic or manual? (reply: 2)
1077. Few colonies with cloning of PCR product after digestion - (reply: 7)
1079. subclone a double-cut insert - (reply: 4)
1053. Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
1055. Is dephosphate needed? - (reply: 6)
1057. Restriction digestion by SpeI and SphI - (reply: 2)
1059. High transformation background in negative control - (reply: 8)
1061. Amplifying a 6.5 kb cDNA - (reply: 3)
1063. pBluescript cloning of PCR products - (reply: 1)
1065. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
1067. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
1069. Reason for white colonies on outer rim of plates - (reply: 9)
1071. SEAP Assay for promoter activity - (reply: 2)
1073. From yeast to mamall cells - principles and methods online? (reply: 7)
1075. E. coli strains ER2566 - characteristic or manual? (reply: 2)
1077. Few colonies with cloning of PCR product after digestion - (reply: 7)
1079. subclone a double-cut insert - (reply: 4)