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1291. Get stuck in a subcoloning: put 0.6kb into 7kb - (reply: 8)
1293. No colony after ligation - (reply: 6)
1295. Cloning a PCR product after digestion - (reply: 7)
1297. troobleshoting for colonies background - (reply: 3)
1299. Silly question concening REs - (reply: 6)
1301. Problem in picking colonies after transformation - (reply: 9)
1303. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
1305. why dephosphoration of vector is not recommended? - (reply: 3)
1307. Positive and negative controls for transformation - What controls to use (reply: 4)
1309. Problems in cloning shRNA - (reply: 13)
1311. dimers of dna (not primers) following digest or pcr? - (reply: 11)
1313. What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
1315. Prepping and transformation - (reply: 2)
1317. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
1319. converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)
1293. No colony after ligation - (reply: 6)
1295. Cloning a PCR product after digestion - (reply: 7)
1297. troobleshoting for colonies background - (reply: 3)
1299. Silly question concening REs - (reply: 6)
1301. Problem in picking colonies after transformation - (reply: 9)
1303. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
1305. why dephosphoration of vector is not recommended? - (reply: 3)
1307. Positive and negative controls for transformation - What controls to use (reply: 4)
1309. Problems in cloning shRNA - (reply: 13)
1311. dimers of dna (not primers) following digest or pcr? - (reply: 11)
1313. What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
1315. Prepping and transformation - (reply: 2)
1317. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
1319. converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)