1651. blue/white screening - problem with recombinant colonies (reply: 1)
1653. Calculate insert/vector ratio for ligation - (reply: 8)
1655. Directional cloning and clone mutations - each clone has a different mutation! (reply: 1)
1657. Cloning issue - vector insert ratio - (reply: 3)
1659. one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end (reply: 13)
1661. cloning large gene - advice (reply: 2)
1663. Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
1665. Transformation problem (HELP!) - (reply: 4)
1667. Help on ligation of big fragment(9kb). - ideas on good ligases? (reply: 2)
1669. QIagen maxi prep - urgent....please help (reply: 4)
1671. cannot isolate plasmid from mutants - please HELP! (reply: 1)
1673. Ligation- what to do if DNA is not concentrated? - (reply: 7)
1675. In frame stop codon! - (reply: 1)
1677. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
1679. problems cloning into plenti6/V5 lentiviral vector - (reply: 2)

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