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571. Isolation of total RNA from different tissues - efficiency? - (reply: 2)
573. PCR screening of colonies - (reply: 3)
575. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
577. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
579. Colony PCR running as smears - (reply: 1)
581. Restriction digested plasmid - (reply: 2)
583. cloning help about pBudCE4.1 - (reply: 1)
585. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
587. cloning problem - (reply: 6)
589. Ligation with T4 DNA Ligase - (reply: 9)
591. Cloning Related Problem - Cloning in pBSK (reply: 1)
593. Problems with directional cloning using BsmBI and Xba I - (reply: 6)
595. cloning problem of big inserts - (reply: 4)
597. plasmid problem - (reply: 3)
599. Colony PCR - (reply: 8)
573. PCR screening of colonies - (reply: 3)
575. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
577. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
579. Colony PCR running as smears - (reply: 1)
581. Restriction digested plasmid - (reply: 2)
583. cloning help about pBudCE4.1 - (reply: 1)
585. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
587. cloning problem - (reply: 6)
589. Ligation with T4 DNA Ligase - (reply: 9)
591. Cloning Related Problem - Cloning in pBSK (reply: 1)
593. Problems with directional cloning using BsmBI and Xba I - (reply: 6)
595. cloning problem of big inserts - (reply: 4)
597. plasmid problem - (reply: 3)
599. Colony PCR - (reply: 8)