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481. route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
483. Cloning PCR product blunt + restriction site - (reply: 2)
485. help in cloning 6kb pcr fragment into topo? - (reply: 5)
487. PCR of lambda phage - (reply: 1)
489. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
491. Gene expression - (reply: 1)
493. Cloning into GATEWAY system - (reply: 3)
495. cloning in expression vector - (reply: 5)
497. Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
499. Getting smear problems in plasmid run - (reply: 8)
501. T4 (without BSA) - How efficient the T4 is? (reply: 2)
503. why the difference in ligation temperature? - (reply: 1)
505. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
507. E.coli promoterless lacZ vector - (reply: 2)
509. klenow/T4 question - Making one end blunt (reply: 4)
483. Cloning PCR product blunt + restriction site - (reply: 2)
485. help in cloning 6kb pcr fragment into topo? - (reply: 5)
487. PCR of lambda phage - (reply: 1)
489. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
491. Gene expression - (reply: 1)
493. Cloning into GATEWAY system - (reply: 3)
495. cloning in expression vector - (reply: 5)
497. Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
499. Getting smear problems in plasmid run - (reply: 8)
501. T4 (without BSA) - How efficient the T4 is? (reply: 2)
503. why the difference in ligation temperature? - (reply: 1)
505. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
507. E.coli promoterless lacZ vector - (reply: 2)
509. klenow/T4 question - Making one end blunt (reply: 4)