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1531. pcDNA 3.1/pTarget and stable cell line - (reply: 10)
1533. Vector databases and resources - (reply: 6)
1535. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
1537. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
1539. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
1541. designing the sequencing primer - (reply: 2)
1543. Me again...on type of polymerase and REs I should use... - (reply: 6)
1545. Cloning - how to avoid religation and no insert? - (reply: 13)
1547. deletion during cloning - (reply: 3)
1549. Strange question regarding DNA in gel-slice... - (reply: 12)
1551. Ligation help needed! - vector religation? (reply: 3)
1553. gene clone - (reply: 1)
1555. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
1557. how to improve ligation efficiency - (reply: 7)
1559. How does IPTG work ? - (reply: 1)
1533. Vector databases and resources - (reply: 6)
1535. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
1537. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
1539. so lost! stupid quenstion about primers.... - designing the primers (reply: 2)
1541. designing the sequencing primer - (reply: 2)
1543. Me again...on type of polymerase and REs I should use... - (reply: 6)
1545. Cloning - how to avoid religation and no insert? - (reply: 13)
1547. deletion during cloning - (reply: 3)
1549. Strange question regarding DNA in gel-slice... - (reply: 12)
1551. Ligation help needed! - vector religation? (reply: 3)
1553. gene clone - (reply: 1)
1555. Which site of NDV NP gene is cut by BamHI and XhoI? - (reply: 2)
1557. how to improve ligation efficiency - (reply: 7)
1559. How does IPTG work ? - (reply: 1)