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1171. Please help! Can Dh5alpha Competent cells be used for electroporation? - (reply: 8)
1173. Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
1175. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
1177. shelf life of siRNA - (reply: 1)
1179. Removing vector restriction sites - and replacing with new ones (reply: 8)
1181. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
1183. what hybridization temp in pcr - (reply: 5)
1185. No positive colony - Help (reply: 4)
1187. Vector problems? - (reply: 7)
1189. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
1191. Calculating insert/vector ratio - (reply: 7)
1193. signal peptide and transmembrane.. - (reply: 1)
1195. klenow fill in protocol for making blunt end - (reply: 5)
1197. Help : no positive colonies - (reply: 19)
1199. insering cDNA before the tag.... - what problems should I consider? (reply: 17)
1173. Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
1175. Expression of GFP fusion: splicing out of tag ? - (reply: 1)
1177. shelf life of siRNA - (reply: 1)
1179. Removing vector restriction sites - and replacing with new ones (reply: 8)
1181. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
1183. what hybridization temp in pcr - (reply: 5)
1185. No positive colony - Help (reply: 4)
1187. Vector problems? - (reply: 7)
1189. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
1191. Calculating insert/vector ratio - (reply: 7)
1193. signal peptide and transmembrane.. - (reply: 1)
1195. klenow fill in protocol for making blunt end - (reply: 5)
1197. Help : no positive colonies - (reply: 19)
1199. insering cDNA before the tag.... - what problems should I consider? (reply: 17)