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721. short DS-oligo cloning - (reply: 2)
723. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
725. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
727. How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
729. urgent HincII digestion problem - (reply: 5)
731. subcloning help urgent! - (reply: 7)
733. primers for promoter amplification - (reply: 1)
735. G418 selection protocol - need ideas for G418 selection (reply: 5)
737. RT-PCR primer designing - (reply: 6)
739. DNA stuck in wells - (reply: 9)
741. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
743. cloning artifact? - (reply: 4)
745. how many colonies to screen? - (reply: 5)
747. PNK treating insert? - (reply: 2)
749. Problem in dephosphorylation - (reply: 7)
723. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
725. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
727. How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
729. urgent HincII digestion problem - (reply: 5)
731. subcloning help urgent! - (reply: 7)
733. primers for promoter amplification - (reply: 1)
735. G418 selection protocol - need ideas for G418 selection (reply: 5)
737. RT-PCR primer designing - (reply: 6)
739. DNA stuck in wells - (reply: 9)
741. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
743. cloning artifact? - (reply: 4)
745. how many colonies to screen? - (reply: 5)
747. PNK treating insert? - (reply: 2)
749. Problem in dephosphorylation - (reply: 7)