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1081. digesting out restriction site - (reply: 6)
1083. Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
1085. in vitro transcription with T7 promoter - (reply: 3)
1087. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
1089. problems with cloning into a pET15b vector - (reply: 2)
1091. Identify insert by restriction digestion - (reply: 6)
1093. VECTOR LIGATION WITH One RE - (reply: 4)
1095. LacZ fusion with pRS415 - Protocol (reply: 2)
1097. LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
1099. PCR: same premix, same everything, different results... - (reply: 21)
1101. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1103. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1105. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1107. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1109. Ligation and cloning failure - (reply: 3)
1083. Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
1085. in vitro transcription with T7 promoter - (reply: 3)
1087. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
1089. problems with cloning into a pET15b vector - (reply: 2)
1091. Identify insert by restriction digestion - (reply: 6)
1093. VECTOR LIGATION WITH One RE - (reply: 4)
1095. LacZ fusion with pRS415 - Protocol (reply: 2)
1097. LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
1099. PCR: same premix, same everything, different results... - (reply: 21)
1101. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1103. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1105. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1107. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1109. Ligation and cloning failure - (reply: 3)