|
811. Digestion, Ligation or transformation problem? - (reply: 8)
812. Directional cloning - (reply: 4)
813. Cloning problems into a big (19 kb) vector - (reply: 10)
814. Promoter experiments - What's next? (reply: 2)
815. Stability of a transgene and its expression - (reply: 3)
816. non radioactive for southern blot - (reply: 3)
817. bacterial transformation - (reply: 4)
818. Promega :Mutagenesis Systems - (reply: 5)
819. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
820. Help with fusion PCR to piece together fragments - (reply: 5)
821. Modification of Vector - (reply: 1)
822. Weird transformation products - (reply: 5)
823. pgh19 Xenopus expression vector - Need sequence (reply: 3)
824. Making more empty vector - (reply: 7)
825. Making Competent Cells - DH5A vs JM109 or anyother (reply: 5)
826. two additional amino acids in gene - (reply: 4)
827. After successful Transfection protein not detectable - (reply: 3)
828. PCR Amplification from clones - (reply: 7)
829. Annealing and cloning of four single stranded oligos - (reply: 1)
830. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
831. how to purify plasmid and genomic dna - (reply: 3)
832. does size matter - how many genes in 1 vector (reply: 10)
833. Religated vector despite a "no insert control" - help! - (reply: 2)
834. insert to vector ratio - (reply: 3)
835. Will self-ligated plasmid run faster than the unligated one? - (reply: 9)
836. PCR cloning, faint band - (reply: 4)
837. BAC Clone Extraction. - (reply: 3)
838. transgene expression - (reply: 3)
839. Ligation into pET28 - (reply: 9)
840. why no insert but can PCR insert out? - (reply: 5)
812. Directional cloning - (reply: 4)
813. Cloning problems into a big (19 kb) vector - (reply: 10)
814. Promoter experiments - What's next? (reply: 2)
815. Stability of a transgene and its expression - (reply: 3)
816. non radioactive for southern blot - (reply: 3)
817. bacterial transformation - (reply: 4)
818. Promega :Mutagenesis Systems - (reply: 5)
819. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
820. Help with fusion PCR to piece together fragments - (reply: 5)
821. Modification of Vector - (reply: 1)
822. Weird transformation products - (reply: 5)
823. pgh19 Xenopus expression vector - Need sequence (reply: 3)
824. Making more empty vector - (reply: 7)
825. Making Competent Cells - DH5A vs JM109 or anyother (reply: 5)
826. two additional amino acids in gene - (reply: 4)
827. After successful Transfection protein not detectable - (reply: 3)
828. PCR Amplification from clones - (reply: 7)
829. Annealing and cloning of four single stranded oligos - (reply: 1)
830. Excision of foreign genes by E.coli - suspicion of attack (reply: 5)
831. how to purify plasmid and genomic dna - (reply: 3)
832. does size matter - how many genes in 1 vector (reply: 10)
833. Religated vector despite a "no insert control" - help! - (reply: 2)
834. insert to vector ratio - (reply: 3)
835. Will self-ligated plasmid run faster than the unligated one? - (reply: 9)
836. PCR cloning, faint band - (reply: 4)
837. BAC Clone Extraction. - (reply: 3)
838. transgene expression - (reply: 3)
839. Ligation into pET28 - (reply: 9)
840. why no insert but can PCR insert out? - (reply: 5)