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1201. how to clone blunt end and sticky end simultaneously? - (reply: 6)
1203. Problem with transformation using electroporator from eppendorf - (reply: 7)
1205. Right Heat-pulse time in transformations - 30, 45, 90 or 3 min?? (reply: 4)
1207. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
1209. During DNA replication - (reply: 6)
1211. is there a restriction about amount of vector in a ligation? - (reply: 14)
1213. Full and Partial Digestion - (reply: 3)
1215. Cloning PCR product - Any ideas? (reply: 3)
1217. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
1219. chromosome length - telomers (reply: 2)
1221. Triple ligation with IRES - (reply: 2)
1223. blunt-end ligation problem - (reply: 3)
1225. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1227. Role of PEG in ligation - (reply: 3)
1229. Basic Question Restriction Digest - (reply: 11)
1203. Problem with transformation using electroporator from eppendorf - (reply: 7)
1205. Right Heat-pulse time in transformations - 30, 45, 90 or 3 min?? (reply: 4)
1207. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
1209. During DNA replication - (reply: 6)
1211. is there a restriction about amount of vector in a ligation? - (reply: 14)
1213. Full and Partial Digestion - (reply: 3)
1215. Cloning PCR product - Any ideas? (reply: 3)
1217. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
1219. chromosome length - telomers (reply: 2)
1221. Triple ligation with IRES - (reply: 2)
1223. blunt-end ligation problem - (reply: 3)
1225. Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
1227. Role of PEG in ligation - (reply: 3)
1229. Basic Question Restriction Digest - (reply: 11)