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1471. [32] phosphorylation hints needed! - (reply: 1)
1473. do primers and dNTPs alter digestion efficiency? - (reply: 4)
1475. is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
1477. cloning 11 kb fragment - (reply: 2)
1479. Sequencing primer dilution - (reply: 2)
1481. plasmid map software? - (reply: 7)
1483. Water or TSB to dilute an over night culture!? - (reply: 6)
1485. Miniprep problem - (reply: 9)
1487. How can I find the sequence after and before my interested ORF? - finding sesequence after and before an ORF? (reply: 4)
1489. Blunt ended ligation - (reply: 7)
1491. what is the best pair of sequencing primer for puc 18 - (reply: 1)
1493. pBR322 relaxed plasmid....what type of media? - (reply: 3)
1495. Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
1497. RNA extraction from blood - (reply: 9)
1499. To inactivate or not to inactivate ligase before transformation? - (reply: 5)
1473. do primers and dNTPs alter digestion efficiency? - (reply: 4)
1475. is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
1477. cloning 11 kb fragment - (reply: 2)
1479. Sequencing primer dilution - (reply: 2)
1481. plasmid map software? - (reply: 7)
1483. Water or TSB to dilute an over night culture!? - (reply: 6)
1485. Miniprep problem - (reply: 9)
1487. How can I find the sequence after and before my interested ORF? - finding sesequence after and before an ORF? (reply: 4)
1489. Blunt ended ligation - (reply: 7)
1491. what is the best pair of sequencing primer for puc 18 - (reply: 1)
1493. pBR322 relaxed plasmid....what type of media? - (reply: 3)
1495. Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
1497. RNA extraction from blood - (reply: 9)
1499. To inactivate or not to inactivate ligase before transformation? - (reply: 5)