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1111. false positives when cloning microsatellite fragments - please help! - (reply: 3)
1112. PCR: same premix, same everything, different results... - (reply: 21)
1113. colony PCR-sometimes works, sometimes doesn't :( - (reply: 3)
1114. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1115. Expression of fusion protein in mammalian cells - (reply: 2)
1116. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1117. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
1118. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1119. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
1120. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1121. Should I purify digested vector before ligation? - (reply: 7)
1122. Ligation and cloning failure - (reply: 3)
1123. restriction direct to ligation? - skip clean up? (reply: 12)
1124. Lipofectamine Transfection of BAECs - (reply: 4)
1125. Competent Cells Preparation - (reply: 9)
1126. Blue and White Colonies - (reply: 3)
1127. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
1128. Problems with Transformation - (reply: 4)
1129. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
1130. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
1131. seeking cloning info - (reply: 9)
1132. The weirdest primer ever - (reply: 7)
1133. problems with Clonetech 10G cells - (reply: 1)
1134. Question on Transcriptional Fusion lacZ - Question on Protocol (reply: 3)
1135. adding resistriction ezymes to primers - cloning (reply: 8)
1136. Problems with gel extraction and ligation - (reply: 12)
1137. forum etiquette for asking for things? - can i scrounge? (reply: 4)
1138. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
1139. more ligation problems! - (reply: 4)
1140. Does gel thickness affect light intensity of the DNA? - (reply: 3)
1112. PCR: same premix, same everything, different results... - (reply: 21)
1113. colony PCR-sometimes works, sometimes doesn't :( - (reply: 3)
1114. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
1115. Expression of fusion protein in mammalian cells - (reply: 2)
1116. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
1117. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
1118. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
1119. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
1120. is there any way to "hide" one restriction site of two in order to dig - (reply: 3)
1121. Should I purify digested vector before ligation? - (reply: 7)
1122. Ligation and cloning failure - (reply: 3)
1123. restriction direct to ligation? - skip clean up? (reply: 12)
1124. Lipofectamine Transfection of BAECs - (reply: 4)
1125. Competent Cells Preparation - (reply: 9)
1126. Blue and White Colonies - (reply: 3)
1127. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
1128. Problems with Transformation - (reply: 4)
1129. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
1130. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
1131. seeking cloning info - (reply: 9)
1132. The weirdest primer ever - (reply: 7)
1133. problems with Clonetech 10G cells - (reply: 1)
1134. Question on Transcriptional Fusion lacZ - Question on Protocol (reply: 3)
1135. adding resistriction ezymes to primers - cloning (reply: 8)
1136. Problems with gel extraction and ligation - (reply: 12)
1137. forum etiquette for asking for things? - can i scrounge? (reply: 4)
1138. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
1139. more ligation problems! - (reply: 4)
1140. Does gel thickness affect light intensity of the DNA? - (reply: 3)