Storage of transformation reaction mix (Promega) - CLoning-transformation (Dec/02/2007 )
How can the remaining transformation mixture (Promega) be stored after using 100ul for plating (post-37c incubation).
Can it be stored at 4c and be reuse for plating after that? How long can it be stored?
Store it at 4°C, it should be fine. I often keep my unplated transformation mixture in the fridge. If I discover after o/n incubation that I need more colonies, I spin down the transformation mixture to concentrate the cells at the bottom, then aspirate most of the media with a pipette. I then briefly vortex the tube to suspend the cells in the remaining volume and plate in on one plate.
Yeh, you could leave it in 4C and use it the next day. On occasions, I have left it at RT, O/N and plated them next day.