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121. question in ligation - (reply: 1)
123. gateway question - pdonr221 (reply: 2)
125. This is the gel pic that is confusing me and my boss - (reply: 7)
127. PEG 4000 for ligation - (reply: 5)
129. Wise expertise needed......Puzzled by something minor..concentration of annealed - (reply: 4)
131. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
133. How long do I incubate after transformation? ? - (reply: 7)
135. Problem with transformation - (reply: 4)
137. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
139. whats the deal with satellite colonies? - (reply: 1)
141. chromosomal DNA extraction E. coli - (reply: 3)
143. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
145. band in -ve rtPCR - (reply: 2)
147. Cloning of 3 kb fragment - (reply: 5)
149. pBLuescript transformation - (reply: 1)
123. gateway question - pdonr221 (reply: 2)
125. This is the gel pic that is confusing me and my boss - (reply: 7)
127. PEG 4000 for ligation - (reply: 5)
129. Wise expertise needed......Puzzled by something minor..concentration of annealed - (reply: 4)
131. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
133. How long do I incubate after transformation? ? - (reply: 7)
135. Problem with transformation - (reply: 4)
137. Combinations of SalI and HindIII - Conditions for double digestion (reply: 4)
139. whats the deal with satellite colonies? - (reply: 1)
141. chromosomal DNA extraction E. coli - (reply: 3)
143. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
145. band in -ve rtPCR - (reply: 2)
147. Cloning of 3 kb fragment - (reply: 5)
149. pBLuescript transformation - (reply: 1)