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211.
Release of an insert from the cloning vector using restriction enzymes -
(reply: 1)
213.
E.coli strain resistant to eco47IR? -
(reply: 1)
215.
pSUPERIOR cloning - please help! -
(reply: 1)
217.
Improving transformation efficiency - large plasmids
(reply: 6)
219.
Questions about PCR amplification using cDNA (RT from total RNA). -
(reply: 6)
221.
dilute the ligation reaction for electroporation -
(reply: 7)
223.
Recombinant PCR -
(reply: 1)
225.
pcr,ligation and transformation with RE SalI and XhoI -
(reply: 4)
227.
cloning into the pBAD-GFPuv -
(reply: 1)
229.
Restriction Enzyme Troubleshooting Help? -
(reply: 2)
231.
PCR and competent cells -
(reply: 3)
233.
How to prohibit BL21 Gold(DE3) plysS autolysis -
(reply: 2)
235.
Taq vs. T4 dna polymerse: difference? -
(reply: 4)
237.
commercial TA cloning vector with Luciferase - cloning promoter sequences
(reply: 2)
239.
How to induce self-ligation of a vector? Self-Circularization? -
(reply: 12)
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