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211. Problems with amplification - (reply: 7)
212. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
213. Large vector self-ligation in presence of insert (1:1)? - (reply: 3)
214. E.coli strain resistant to eco47IR? - (reply: 1)
215. How to find plasmid containing 35S and Nos ? - (reply: 3)
216. pSUPERIOR cloning - please help! - (reply: 1)
217. Need to sequence gel purified vectors exposed to UV? - (reply: 2)
218. Improving transformation efficiency - large plasmids (reply: 6)
219. Molecular Cloning - Subcloning (reply: 3)
220. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
221. mutations in primer sequence - (reply: 2)
222. dilute the ligation reaction for electroporation - (reply: 7)
223. digestion of pcr - (reply: 2)
224. Recombinant PCR - (reply: 1)
225. Plasmid lost during culture - (reply: 19)
226. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
227. double digestion and ligation - (reply: 3)
228. cloning into the pBAD-GFPuv - (reply: 1)
229. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
230. Restriction Enzyme Troubleshooting Help? - (reply: 2)
231. Ligation: time and temperature - (reply: 8)
232. PCR and competent cells - (reply: 3)
233. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
234. How to prohibit BL21 Gold(DE3) plysS autolysis - (reply: 2)
235. Help restriction analysis of plasmid - (reply: 11)
236. Taq vs. T4 dna polymerse: difference? - (reply: 4)
237. Can I store plasmid in 4C refrigerator? - (reply: 11)
238. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
239. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
240. How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)
212. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
213. Large vector self-ligation in presence of insert (1:1)? - (reply: 3)
214. E.coli strain resistant to eco47IR? - (reply: 1)
215. How to find plasmid containing 35S and Nos ? - (reply: 3)
216. pSUPERIOR cloning - please help! - (reply: 1)
217. Need to sequence gel purified vectors exposed to UV? - (reply: 2)
218. Improving transformation efficiency - large plasmids (reply: 6)
219. Molecular Cloning - Subcloning (reply: 3)
220. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
221. mutations in primer sequence - (reply: 2)
222. dilute the ligation reaction for electroporation - (reply: 7)
223. digestion of pcr - (reply: 2)
224. Recombinant PCR - (reply: 1)
225. Plasmid lost during culture - (reply: 19)
226. pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
227. double digestion and ligation - (reply: 3)
228. cloning into the pBAD-GFPuv - (reply: 1)
229. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
230. Restriction Enzyme Troubleshooting Help? - (reply: 2)
231. Ligation: time and temperature - (reply: 8)
232. PCR and competent cells - (reply: 3)
233. Suddenly no colonies, can cells 'go off' in -80? - (reply: 1)
234. How to prohibit BL21 Gold(DE3) plysS autolysis - (reply: 2)
235. Help restriction analysis of plasmid - (reply: 11)
236. Taq vs. T4 dna polymerse: difference? - (reply: 4)
237. Can I store plasmid in 4C refrigerator? - (reply: 11)
238. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
239. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
240. How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)