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1681. Double digestion of PCR product and ligation - (reply: 5)
1683. adding A overhangs - how much dATP? (reply: 4)
1685. cloning problem - sticky end cloning with PCR insert - (reply: 3)
1687. subcloning into a single NotI site - (reply: 11)
1689. How to construct T vector - (reply: 8)
1691. Dephosphorylation of vector before ligation - Is it required? (reply: 38)
1693. need help in molecular cloning - molecular biology (reply: 6)
1695. cloning from PCR - (reply: 25)
1697. blunt-cloning - all are vector self-ligation products? (reply: 33)
1699. plasmid can't be digested by enzyme - (reply: 13)
1683. adding A overhangs - how much dATP? (reply: 4)
1685. cloning problem - sticky end cloning with PCR insert - (reply: 3)
1687. subcloning into a single NotI site - (reply: 11)
1689. How to construct T vector - (reply: 8)
1691. Dephosphorylation of vector before ligation - Is it required? (reply: 38)
1693. need help in molecular cloning - molecular biology (reply: 6)
1695. cloning from PCR - (reply: 25)
1697. blunt-cloning - all are vector self-ligation products? (reply: 33)
1699. plasmid can't be digested by enzyme - (reply: 13)