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751. DNA stuck in wells - (reply: 9)
753. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
755. cloning artifact? - (reply: 4)
757. how many colonies to screen? - (reply: 5)
759. PNK treating insert? - (reply: 2)
761. Problem in dephosphorylation - (reply: 7)
763. Destruction of restriction site - (reply: 1)
765. TA-cloning vector contamination - (reply: 3)
767. Vector with two promoters - Molecular Biology (reply: 2)
769. Contamination from satellite colonies - (reply: 1)
771. Transformation-Min plasmid reqd - (reply: 3)
773. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
775. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
777. blunt end ligation problem - (reply: 4)
779. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
753. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
755. cloning artifact? - (reply: 4)
757. how many colonies to screen? - (reply: 5)
759. PNK treating insert? - (reply: 2)
761. Problem in dephosphorylation - (reply: 7)
763. Destruction of restriction site - (reply: 1)
765. TA-cloning vector contamination - (reply: 3)
767. Vector with two promoters - Molecular Biology (reply: 2)
769. Contamination from satellite colonies - (reply: 1)
771. Transformation-Min plasmid reqd - (reply: 3)
773. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
775. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
777. blunt end ligation problem - (reply: 4)
779. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)