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1261.
Making blunt and stick end vector -
(reply: 2)
1263.
Ligation worked - maybe? - Aimikins - I've got colonies! Which size do I pick?
(reply: 4)
1265.
Which Klenow product to use?! - With or without exo-nuclease activity?
(reply: 5)
1267.
Cloning of long gene -
(reply: 1)
1269.
Cutting close to DNA ends -
(reply: 6)
1271.
basal expression - Is it possible in DH5a or in Rosetta pLYS? -
(reply: 3)
1273.
toxic gene ligation/transformation URGENT HELP! -
(reply: 1)
1275.
Is OVER-dephosphorylation possible? -
(reply: 2)
1277.
Reusing Qiagen Tips -
(reply: 2)
1279.
problem in ligation and transformation -
(reply: 18)
1281.
Ethanol precipitation problem -
(reply: 7)
1283.
cloning restriction fragment for sequencing -
(reply: 1)
1285.
How do I check frame? -
(reply: 3)
1287.
Many colonies but no positive clone -
(reply: 1)
1289.
plasmid sequencing -
(reply: 4)
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