SV40-less Luciferase promoter plasmid? - (Aug/22/2005 )
I'm trying to do a luciferase reporter to test out an enhancer sequence. Unfortunately, the SV40 promoter in the pGL3 that I normally use contains binding sites for the protein that should bind to my enhancer, so I can't use that. Does anyone know of any other luciferase (or other reporter) promoter plasmids I could use instead?
-post-dog-
hi there,
try new series of Promega vectors, e.g. pGL4, they seems better then PgL3 vectors and have less sites for transcription factors.
maybe that could solve your problem.
-sushina-
if you are happy to use Renilla luciferase rather than firefly there are more options
You can look at Promega's phRL series - these have luciferase under the control of things like the HSV-TK promoter or the CMV promoter, etc.
-Doc_Martin-