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Q re: optimizing sonication in ChIP - (May/26/2005 )

Do I need to purify the sample after de-crosslinking it? I did it before, but now think about it - Not really necessary, because the proteins shouldn't show up in my agarose gel. Is it correct?

-koira-

no, proteins will still be aroound.

perform standard phenol chlorofom isolation of DNA after decross linking as if you don't it may inhibit downstream experiments.

good luck

Nick

-methylnick-

Thanks Nick.

-koira-