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HELP! Background in ChIP assay - (Apr/05/2006 )

I have been trying to remove residual background from ChIP experiments for a while now. I have tried multiple washes after IP. I have been getting equal PCR products in my mock IPs and antibody samples.

Please if anyone has any suggestions, help me out.



Have you tried pre-blocking your agarose beads. If not, please read this thread