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High quality formaldehyde for ChIP? - (Mar/25/2008 )

I'm using the Upstate kit for ChIP and they say to use "high-quality" formaldehyde. What constitutes high-quality? I've been using a 37% stock solution which seems to be commonly suggested in ChIP protocols even though I would never use such a thing for immunofluorescence. I'm using Fisher F79-500 at the moment. I checked the bottle and it contains a rather large percentage of methanol, which also seems problematic to me. I'm happy to make fresh from paraformaldehyde, but if it is not necessary then I'd rather stick to what everyone else finds suitable.

Is there a particular grade of formaldehyde that people use? Catalog number?

thanks

-kman42-

QUOTE (kman42 @ Mar 25 2008, 01:01 PM)
I'm using the Upstate kit for ChIP and they say to use "high-quality" formaldehyde. What constitutes high-quality? I've been using a 37% stock solution which seems to be commonly suggested in ChIP protocols even though I would never use such a thing for immunofluorescence. I'm using Fisher F79-500 at the moment. I checked the bottle and it contains a rather large percentage of methanol, which also seems problematic to me. I'm happy to make fresh from paraformaldehyde, but if it is not necessary then I'd rather stick to what everyone else finds suitable.

Is there a particular grade of formaldehyde that people use? Catalog number?

thanks



Anyone?

Any info on this would be appreciated.

-kman42-

QUOTE (kman42 @ Apr 4 2008, 10:31 AM)
QUOTE (kman42 @ Mar 25 2008, 01:01 PM)
I'm using the Upstate kit for ChIP and they say to use "high-quality" formaldehyde. What constitutes high-quality? I've been using a 37% stock solution which seems to be commonly suggested in ChIP protocols even though I would never use such a thing for immunofluorescence. I'm using Fisher F79-500 at the moment. I checked the bottle and it contains a rather large percentage of methanol, which also seems problematic to me. I'm happy to make fresh from paraformaldehyde, but if it is not necessary then I'd rather stick to what everyone else finds suitable.

Is there a particular grade of formaldehyde that people use? Catalog number?

thanks



Anyone?

Any info on this would be appreciated.


I use the 37% stock stablized with methanol that everyone uses. I haven't heard of anyone having to use the ampules or making it fresh from paraformaldehyde. The 37% stock is probably good enough because you're not trying to preserve to many structures, just the links between protein and DNA.

-KPDE-