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How do people shear their chromatin for ChIP? - Tips and Hints with Fragmenting Chromatin (Jan/29/2007 )

Sorry I just started as an honours student I don't really know the answer to your questions but may i ask how exactly did u prepare the DNA to a reasonable size, eg less than 1kb for the Chromatin IP?


We have a bioruptor water bath sonicator to shear chromatin.

15 mins, 30 second pulses on high at 4C