Magna ChIP kit from Upstate - Insufficient volume or reagents? (Feb/11/2008 )

Heya,

I've bought the Magna ChIP kit from Upstate and I'm sure others out there are using it. I've noticed that there is not enough of the cell lysis and nuclear lysis buffers to cover the 22 assays the kit is claiming it can handle. 500ul is required for each sample yet only 5ml is supplied. I've contacted the company but haven't been able to come up with any answers as to why this is.

Has anyone else out there used it and know what it going on? Does anyone have their own recipe for these buffers which have worked??

I understand ChIP is a tough method without having not enough reagents!

Dave

It looks as though I can answer my own question!

The Magna ChIP kit says that it has all the reagents necessary to perform 22 immunoprecipitation assays. While this is true it is also misleading... You can perform 22 assays, but not 22 separate populations of cells. ie at any point you need to peform at least 2 immunoprecipitations (which is probably the minimum you'd do anyway) because only enough cell lysis buffer (and other reagents) is supplied to handle 10 plates of cells.

Just thought I'd let you know incase anyone was considering to use the kit.

Dave