ChIP control problems - (Jul/14/2006 )
if doing ChIP following upstate protocol, I need to perform an independent assay for no-ab control? or I could divide our one assay sample into two parts for Ip and mocking.
the upstate protocol said only 1% (20ul) of diluted cell supernatant is enough for Input control. every guys did like this? or add more for input control.
-wop168-
QUOTE (wop168 @ Jul 14 2006, 10:49 AM)
if doing ChIP following upstate protocol, I need to perform an independent assay for no-ab control? or I could divide our one assay sample into two parts for Ip and mocking.
the upstate protocol said only 1% (20ul) of diluted cell supernatant is enough for Input control. every guys did like this? or add more for input control.
the upstate protocol said only 1% (20ul) of diluted cell supernatant is enough for Input control. every guys did like this? or add more for input control.
I divide all my samples for anti-tag Ab (people prefere somethimes to use an anti-tag Ab instead of No Ab) and Ab of interest.
For input chromatin, I use 10ul out of 240, that means 4% of the total Chromatin. I can tell you that it's more than enough...
-dnafactory-