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CHIP with nuclei - (Mar/18/2005 )

Hello,

Can CHIP assay be performed using nuclei as its all about chromatin?

-nidhi-

Hi Nidhi,

of course you can perform CHIP with nuclei, though you will have to treat the nuclei by either sonication or micrococcal nuclease digest to fragment the nuclei to a size that can be precipitated with the antibody you use for CHIP.

Good luck!

Nick

-methylnick-