Dynal (Magnetic) beads for ChIP? - (Oct/18/2006 )

Hi everyone,

I was wondering if any of you out there have used Dynal Magnetic beads (such as protein A Dynabeads) to do the IP for your ChIP assay. I'm still getting some background amplification with my IgG control or beads alone control so I'm looking for anything to reduce the background. I am currently using the salmon sperm DNA protein A agarose from Upstate. If you haveused the Dynabeads, please let me know either way how it works for you.

Thank you.
Sonixchip

-sonixchip-

QUOTE (sonixchip @ Oct 18 2006, 10:54 PM)

I had little success with both ptn-A-agarose and dynabeads. But i would definately say the dynabeads were better for reducing non spcific signal. Try optimising the amout you use (i started at 5ul per IP and ended up on 2ul).

-aadamson81-

QUOTE (aadamson81 @ Oct 20 2006, 10:26 AM)

QUOTE (sonixchip @ Oct 18 2006, 10:54 PM)

Thanks for your reply. I will definitely give it a try.

-sonixchip-

Dynabeads are nice to handle; try also Dynabeads-labeled secondary Ab; important, clear your sample before ChIP with Dynabeads-protein A/G or Dynabeads-secondary Ab

-The Bearer-