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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
152. how do GST-tags affect gel filtration results? - (reply: 5)
153. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
154. Stable protein expression in Vero cells - (reply: 2)
155. Which kind of ATP do you usually use in you experiment? - (reply: 2)
156. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
157. GST-tagged protein expression problem! :-( - (reply: 4)
158. Better (quicker) way to pipet glycerol? - (reply: 3)
159. How OD600 influence on IPTG induction and protein expression - (reply: 5)
160. does EGFP dimerize? - (reply: 3)
161. IPTG induction doesn't work:( - (reply: 3)
162. question about transformation - (reply: 1)
163. Mouse IgG1 purification - Protein G - (reply: 1)
164. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
165. what will happen if you let your affinity column beads dry? - (reply: 1)
166. pichia epression problem - (reply: 1)
167. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
168. Multiple protein bands after expression - (reply: 6)
169. his-tag prufication lysis problem:( - (reply: 4)
170. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
171. co-eluted protein with his-tag protein - (reply: 1)
172. Help with His-Tag protein purification - (reply: 2)
173. Discrepancy between tissue and cell culture - (reply: 1)
174. Expression and Lysozyme - (reply: 3)
175. Rosetta-gami B Incubation Time - (reply: 1)
176. Decreased protein yields after successive passaging? - (reply: 1)
177. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
178. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
179. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
180. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)

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