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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. High gravity crush beads ? - (reply: 1)
152. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
153. how do GST-tags affect gel filtration results? - (reply: 5)
154. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
155. Stable protein expression in Vero cells - (reply: 2)
156. Which kind of ATP do you usually use in you experiment? - (reply: 2)
157. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
158. GST-tagged protein expression problem! :-( - (reply: 4)
159. Better (quicker) way to pipet glycerol? - (reply: 3)
160. How OD600 influence on IPTG induction and protein expression - (reply: 5)
161. does EGFP dimerize? - (reply: 3)
162. IPTG induction doesn't work:( - (reply: 3)
163. question about transformation - (reply: 1)
164. Mouse IgG1 purification - Protein G - (reply: 1)
165. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
166. what will happen if you let your affinity column beads dry? - (reply: 1)
167. pichia epression problem - (reply: 1)
168. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
169. Multiple protein bands after expression - (reply: 6)
170. his-tag prufication lysis problem:( - (reply: 4)
171. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
172. co-eluted protein with his-tag protein - (reply: 1)
173. Help with His-Tag protein purification - (reply: 2)
174. Discrepancy between tissue and cell culture - (reply: 1)
175. Expression and Lysozyme - (reply: 3)
176. Rosetta-gami B Incubation Time - (reply: 1)
177. Decreased protein yields after successive passaging? - (reply: 1)
178. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
179. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
180. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)

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