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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. Problems with overnights - (reply: 5)
152. problem in extracting the protein - (reply: 1)
153. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
154. Protein Purification from Polyacrylamide Gels - (reply: 3)
155. Protein Expression vector with small tag - (reply: 1)
156. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
157. Albumin Removal From BSA - (reply: 1)
158. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
159. Insulin production - (reply: 2)
160. High Yield Protein Expression 10g/L - (reply: 5)
161. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
162. GST- tagged protein expressed in insoluble - (reply: 5)
163. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
164. Prepare protein standards for concentration determination - (reply: 1)
165. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
166. Tagged recombinant protein has different mobility? - (reply: 4)
167. My protein doesn't bind to the Ni-NTA column - (reply: 11)
168. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
169. TCA IgG precipitation - (reply: 2)
170. Does triple labeled protein precipitate more easily? - (reply: 2)
171. High gravity crush beads ? - (reply: 1)
172. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
173. how do GST-tags affect gel filtration results? - (reply: 5)
174. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
175. Stable protein expression in Vero cells - (reply: 2)
176. Which kind of ATP do you usually use in you experiment? - (reply: 2)
177. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
178. GST-tagged protein expression problem! :-( - (reply: 4)
179. Better (quicker) way to pipet glycerol? - (reply: 3)
180. How OD600 influence on IPTG induction and protein expression - (reply: 5)

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