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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
152. Albumin Removal From BSA - (reply: 1)
153. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
154. Insulin production - (reply: 2)
155. High Yield Protein Expression 10g/L - (reply: 5)
156. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
157. GST- tagged protein expressed in insoluble - (reply: 5)
158. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
159. Prepare protein standards for concentration determination - (reply: 1)
160. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
161. Tagged recombinant protein has different mobility? - (reply: 4)
162. My protein doesn't bind to the Ni-NTA column - (reply: 11)
163. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
164. TCA IgG precipitation - (reply: 2)
165. Does triple labeled protein precipitate more easily? - (reply: 2)
166. High gravity crush beads ? - (reply: 1)
167. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
168. how do GST-tags affect gel filtration results? - (reply: 5)
169. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
170. Stable protein expression in Vero cells - (reply: 2)
171. Which kind of ATP do you usually use in you experiment? - (reply: 2)
172. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
173. GST-tagged protein expression problem! :-( - (reply: 4)
174. Better (quicker) way to pipet glycerol? - (reply: 3)
175. How OD600 influence on IPTG induction and protein expression - (reply: 5)
176. does EGFP dimerize? - (reply: 3)
177. IPTG induction doesn't work:( - (reply: 3)
178. question about transformation - (reply: 1)
179. Mouse IgG1 purification - Protein G - (reply: 1)
180. Make my own BL21-CodonPlus(DE3)? - (reply: 3)

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