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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
152. 1M IPTG left for 12-14h @ Room temp - still good? - (reply: 2)
153. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
154. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
155. Desalting resin - (reply: 1)
156. RNA extraction by trizol - (reply: 3)
157. Problems in expression of target protein in E. coli - (reply: 3)
158. Membrane protein extraction - (reply: 2)
159. Is it possible to use wireless connection to control and monitor FPLC? - (reply: 3)
160. EMSA - general question - (reply: 1)
161. Understanding immunoprecipitation - where do the beads go? - (reply: 2)
162. Soluble protien of GST - (reply: 2)
163. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
164. Contaminants in protein purification.....help! - (reply: 3)
165. Protein expression and purification - Please help - (reply: 2)
166. Protein purifcation without chromatography - (reply: 6)
167. Bacterial Media Formulation - (reply: 2)
168. tag for protein purification - (reply: 7)
169. Protein is not Binding to DE52 column material - (reply: 2)
170. expression problem with pBAD33...similar experience? - (reply: 2)
171. MBP-Tagged protein degrading after expression - (reply: 1)
172. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
173. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
174. Controls for co-immunoprecipitation - (reply: 3)
175. How to improve enzyme activity? - (reply: 2)
176. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
177. Sediments/debris/pellet in protein lysate? - (reply: 3)
178. Cleavage of GST-tag with Factor Xa - (reply: 1)
179. Trouble in GST- fusion protein purification - (reply: 1)
180. Helppurification of expressed protein - (reply: 2)

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