Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
152. GST- tagged protein expressed in insoluble - (reply: 5)
153. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
154. Prepare protein standards for concentration determination - (reply: 1)
155. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
156. Tagged recombinant protein has different mobility? - (reply: 4)
157. My protein doesn't bind to the Ni-NTA column - (reply: 11)
158. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
159. TCA IgG precipitation - (reply: 2)
160. Does triple labeled protein precipitate more easily? - (reply: 2)
161. High gravity crush beads ? - (reply: 1)
162. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
163. how do GST-tags affect gel filtration results? - (reply: 5)
164. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
165. Stable protein expression in Vero cells - (reply: 2)
166. Which kind of ATP do you usually use in you experiment? - (reply: 2)
167. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
168. GST-tagged protein expression problem! :-( - (reply: 4)
169. Better (quicker) way to pipet glycerol? - (reply: 3)
170. How OD600 influence on IPTG induction and protein expression - (reply: 5)
171. does EGFP dimerize? - (reply: 3)
172. IPTG induction doesn't work:( - (reply: 3)
173. question about transformation - (reply: 1)
174. Mouse IgG1 purification - Protein G - (reply: 1)
175. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
176. what will happen if you let your affinity column beads dry? - (reply: 1)
177. pichia epression problem - (reply: 1)
178. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
179. Multiple protein bands after expression - (reply: 6)
180. his-tag prufication lysis problem:( - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20