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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty - (reply: 4)
152. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
153. Desalting resin - (reply: 1)
154. RNA extraction by trizol - (reply: 3)
155. Problems in expression of target protein in E. coli - (reply: 3)
156. Membrane protein extraction - (reply: 2)
157. Is it possible to use wireless connection to control and monitor FPLC? - (reply: 3)
158. EMSA - general question - (reply: 1)
159. Understanding immunoprecipitation - where do the beads go? - (reply: 2)
160. Soluble protien of GST - (reply: 2)
161. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
162. Contaminants in protein purification.....help! - (reply: 3)
163. Protein expression and purification - Please help - (reply: 2)
164. Protein purifcation without chromatography - (reply: 6)
165. Bacterial Media Formulation - (reply: 2)
166. tag for protein purification - (reply: 7)
167. Protein is not Binding to DE52 column material - (reply: 2)
168. expression problem with pBAD33...similar experience? - (reply: 2)
169. MBP-Tagged protein degrading after expression - (reply: 1)
170. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
171. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
172. Controls for co-immunoprecipitation - (reply: 3)
173. How to improve enzyme activity? - (reply: 2)
174. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
175. Sediments/debris/pellet in protein lysate? - (reply: 3)
176. Cleavage of GST-tag with Factor Xa - (reply: 1)
177. Trouble in GST- fusion protein purification - (reply: 1)
178. Helppurification of expressed protein - (reply: 2)
179. protein expression by changing start codon - (reply: 1)
180. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)

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