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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. Tagged recombinant protein has different mobility? - (reply: 4)
152. My protein doesn't bind to the Ni-NTA column - (reply: 11)
153. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
154. TCA IgG precipitation - (reply: 2)
155. Does triple labeled protein precipitate more easily? - (reply: 2)
156. High gravity crush beads ? - (reply: 1)
157. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
158. how do GST-tags affect gel filtration results? - (reply: 5)
159. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
160. Stable protein expression in Vero cells - (reply: 2)
161. Which kind of ATP do you usually use in you experiment? - (reply: 2)
162. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
163. GST-tagged protein expression problem! :-( - (reply: 4)
164. Better (quicker) way to pipet glycerol? - (reply: 3)
165. How OD600 influence on IPTG induction and protein expression - (reply: 5)
166. does EGFP dimerize? - (reply: 3)
167. IPTG induction doesn't work:( - (reply: 3)
168. question about transformation - (reply: 1)
169. Mouse IgG1 purification - Protein G - (reply: 1)
170. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
171. what will happen if you let your affinity column beads dry? - (reply: 1)
172. pichia epression problem - (reply: 1)
173. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
174. Multiple protein bands after expression - (reply: 6)
175. his-tag prufication lysis problem:( - (reply: 4)
176. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
177. co-eluted protein with his-tag protein - (reply: 1)
178. Help with His-Tag protein purification - (reply: 2)
179. Discrepancy between tissue and cell culture - (reply: 1)
180. Expression and Lysozyme - (reply: 3)

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