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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
151. How OD600 influence on IPTG induction and protein expression - (reply: 5)
152. does EGFP dimerize? - (reply: 3)
153. IPTG induction doesn't work:( - (reply: 3)
154. question about transformation - (reply: 1)
155. Mouse IgG1 purification - Protein G - (reply: 1)
156. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
157. what will happen if you let your affinity column beads dry? - (reply: 1)
158. pichia epression problem - (reply: 1)
159. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
160. Multiple protein bands after expression - (reply: 6)
161. his-tag prufication lysis problem:( - (reply: 4)
162. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
163. co-eluted protein with his-tag protein - (reply: 1)
164. Help with His-Tag protein purification - (reply: 2)
165. Discrepancy between tissue and cell culture - (reply: 1)
166. Expression and Lysozyme - (reply: 3)
167. Rosetta-gami B Incubation Time - (reply: 1)
168. Decreased protein yields after successive passaging? - (reply: 1)
169. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
170. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
171. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
172. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
173. how much time for prepared gel to keep bfore loading? - (reply: 5)
174. plant extraction procedure for protein - (reply: 3)
175. brain tissue homogenization with sample buffer?? - (reply: 2)
176. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
177. Reversed-Phase chromatography for protein purification - (reply: 3)
178. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
179. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
180. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)

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