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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
601. Low level of expression - in pASK-IBA7 vector (reply: 6)
602. dnase treatment before his-tag purification - (reply: 3)
603. protein aggregation problem - (reply: 3)
604. protein precipitates out during concentrating it - (reply: 2)
605. how to put in evidence protein after IP - (reply: 2)
606. GST-fusion protein purification - How to purify it? (reply: 9)
607. IPTG - (reply: 2)
608. Protein lysis buffer - (reply: 4)
609. IP low protein and MS - (reply: 1)
610. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
611. Microcon troubles - Very low recovery (reply: 4)
612. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
613. Antibody-beads crosslink - troubles (reply: 5)
614. What is stringent vector? - Is it related to level of expression? (reply: 2)
615. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
616. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
617. Concentrating Protein - protein precipitates upon concentrating (reply: 1)
618. Endogenous protein purification - (reply: 2)
619. Protein in and out of solution - (reply: 1)
620. Protein extraction from rat aorta - (reply: 3)
621. heterodimer - bicistronic vector? (reply: 2)
622. stripped Ni column turn brown yellow - (reply: 1)
623. Making Recombinant Proteins - (reply: 2)
624. GST- protein, batch purification - no protein after elution (reply: 3)
625. Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
626. Insoluble TEV Protease - (reply: 2)
627. How to elute this protein?! - (reply: 5)
628. Recombinant protein or peptide? - (reply: 1)
629. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
630. pqe30 - (reply: 1)

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