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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
601. IP low protein and MS - (reply: 1)
602. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
603. Microcon troubles - Very low recovery (reply: 4)
604. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
605. Antibody-beads crosslink - troubles (reply: 5)
606. What is stringent vector? - Is it related to level of expression? (reply: 2)
607. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
608. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
609. Concentrating Protein - protein precipitates upon concentrating (reply: 1)
610. Endogenous protein purification - (reply: 2)
611. Protein in and out of solution - (reply: 1)
612. Protein extraction from rat aorta - (reply: 3)
613. heterodimer - bicistronic vector? (reply: 2)
614. stripped Ni column turn brown yellow - (reply: 1)
615. Making Recombinant Proteins - (reply: 2)
616. GST- protein, batch purification - no protein after elution (reply: 3)
617. Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
618. Insoluble TEV Protease - (reply: 2)
619. How to elute this protein?! - (reply: 5)
620. Recombinant protein or peptide? - (reply: 1)
621. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
622. pqe30 - (reply: 1)
623. truncation of protein expression - (reply: 1)
624. GST fusion protein purification - (reply: 1)
625. unstable protein expression - (reply: 1)
626. B-per GST fusion protein purification kit - (reply: 2)
627. Protein precipitates out so fast - (reply: 5)
628. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
629. Lysis Buffer for Total Protein Assay - (reply: 1)
630. primer design for pBAD topo expression kit - (reply: 2)

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