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New Forum Archives (2009-): :
271. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
273. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
275. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
277. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
279. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
281. Weak affinity chromatography - (reply: 2)
283. e coli vector and host selection - (reply: 1)
285. Purifying recombinant protein from pellet? - (reply: 2)
287. Protein ladder - (reply: 1)
289. Can anyone help me to identify what line is that??? - (reply: 4)
291. membrane protein expression - (reply: 1)
293. Protease Inhibitor Cocktails - (reply: 3)
295. Extra bands after protein expression/purification - (reply: 4)
297. Expression of protein with pI 8.2 - (reply: 1)
299. Alternatives to benzonase? - (reply: 1)
273. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
275. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
277. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
279. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
281. Weak affinity chromatography - (reply: 2)
283. e coli vector and host selection - (reply: 1)
285. Purifying recombinant protein from pellet? - (reply: 2)
287. Protein ladder - (reply: 1)
289. Can anyone help me to identify what line is that??? - (reply: 4)
291. membrane protein expression - (reply: 1)
293. Protease Inhibitor Cocktails - (reply: 3)
295. Extra bands after protein expression/purification - (reply: 4)
297. Expression of protein with pI 8.2 - (reply: 1)
299. Alternatives to benzonase? - (reply: 1)