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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
271. Low or even None expression - (reply: 1)
272. How can I detect mannosidase activity of a protein - (reply: 1)
273. Protein Aggregation with 6xHis tagged CreGFP - (reply: 1)
274. Protein stability at room temperature - (reply: 3)
275. Testing Solubility in different pH's and salt concentrations for NMR. - (reply: 2)
276. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
277. Protein purification from HEK 293 cells - (reply: 1)
278. Upscaling protein expression in E.coli - (reply: 2)
279. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
280. nuclease contamination in protein prep - (reply: 4)
281. Problems Expressing from Kluyveromyces lactis - (reply: 1)
282. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
283. Unable to Purify protein! - Protein purification (reply: 2)
284. Problem with Pierce GST spin purification column - (reply: 6)
285. HIS-tagged protein purification - (reply: 12)
286. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
287. His-tagged protein purification problem - (reply: 2)
288. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
289. Signal Peptides - To leave or not to leave (reply: 2)
290. Low expression of pET21a construct - (reply: 6)
291. GST-fusion protein degrades in-vivo - (reply: 3)
292. Inclusion body purification - (reply: 1)
293. help with protein elution - (reply: 1)
294. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
295. Active protein extract from corn - (reply: 2)
296. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
297. Reduced Glutathione affects thrombin activity? - (reply: 2)
298. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
299. Should I worry about endotoxin? - (reply: 4)
300. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)

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