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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. RNA immunoprecipitation...HELP! - (reply: 4)
422. Dialysis - A question about buffers (reply: 6)
423. Purification problems his-tag - I have tried everything (reply: 3)
424. GST-tagged protein binds to column but is not eluted - (reply: 1)
425. urea-denatured protein precipitates after dialysis - (reply: 1)
426. Expressing 4 proteins in an operon - (reply: 6)
427. More His-tag problems - (reply: 4)
428. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
429. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
430. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
431. Protein precipitates upon dialysis - (reply: 5)
432. membrane protein aggregation - (reply: 1)
433. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
434. His Tag Protein Purification - (reply: 6)
435. Amylose bound protein elution - (reply: 1)
436. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
437. Soluble aggregates - (reply: 3)
438. insect protein expression - (reply: 1)
439. Can you store TNT translation products? - (reply: 1)
440. How to pack the FPLC column - (reply: 2)
441. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
442. Sonication issues! - (reply: 3)
443. complete gene for expression? - (reply: 2)
444. buffer for membrane protein - (reply: 1)
445. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
446. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
447. membrane protein purification - (reply: 6)
448. Protein expression failing - (reply: 1)
449. How does aggregation influence absorbtion at 280nm? - (reply: 1)
450. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)

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