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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. to get protein in cytosol from inclusion bodies - (reply: 4)
422. Inclusion bodies in e.coli - (reply: 1)
423. lysis of e.coli by sonication - (reply: 2)
424. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
425. Protein expression - (reply: 8)
426. problem with ion exchange puification - protein not binding (reply: 3)
427. Protein Purification by precipitation - (reply: 2)
428. refolding proteins using dialysis - (reply: 1)
429. protein refolding - (reply: 1)
430. Zeocine resistence, pichia pastoris - (reply: 3)
431. Problem with MBP and GST tag purification - (reply: 1)
432. concentrating protein by method other than tff - (reply: 1)
433. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
434. M2 Flag purification - (reply: 2)
435. Quantitating IP'd proteins - (reply: 1)
436. Removal of Tightly Bound Nucleic Acids - (reply: 3)
437. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
438. Recombinant protein aggregation after purification - (reply: 2)
439. average total protein content per 293t cell - (reply: 2)
440. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
441. expression of MBP fusion protein - leaky and very low yield (reply: 2)
442. Calmodulin binding assay - (reply: 1)
443. strange problem with His purification - (reply: 1)
444. E.coli expression - (reply: 5)
445. why my protein precipitates? - (reply: 2)
446. DTT in GST elution buffer - (reply: 1)
447. vectors and his-tags - (reply: 3)
448. SDS boiled samples - (reply: 1)
449. E coli lysis by lysozyme - (reply: 2)
450. His-tag protein expression - (reply: 1)

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