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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
422. RNA immunoprecipitation...HELP! - (reply: 4)
423. Dialysis - A question about buffers (reply: 6)
424. Purification problems his-tag - I have tried everything (reply: 3)
425. GST-tagged protein binds to column but is not eluted - (reply: 1)
426. urea-denatured protein precipitates after dialysis - (reply: 1)
427. Expressing 4 proteins in an operon - (reply: 6)
428. More His-tag problems - (reply: 4)
429. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
430. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
431. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
432. Protein precipitates upon dialysis - (reply: 5)
433. membrane protein aggregation - (reply: 1)
434. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
435. His Tag Protein Purification - (reply: 6)
436. Amylose bound protein elution - (reply: 1)
437. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
438. Soluble aggregates - (reply: 3)
439. insect protein expression - (reply: 1)
440. Can you store TNT translation products? - (reply: 1)
441. How to pack the FPLC column - (reply: 2)
442. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
443. Sonication issues! - (reply: 3)
444. complete gene for expression? - (reply: 2)
445. buffer for membrane protein - (reply: 1)
446. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
447. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
448. membrane protein purification - (reply: 6)
449. Protein expression failing - (reply: 1)
450. How does aggregation influence absorbtion at 280nm? - (reply: 1)

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