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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
422. His-tag protein lost it's tag - (reply: 2)
423. AnionExchange problem: proteins do not bind - (reply: 12)
424. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
425. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
426. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
427. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
428. IMPACT system purification problem - (reply: 1)
429. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
430. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
431. How to normalize my samples before I transfect my cells - (reply: 6)
432. FBS interference in SDS PAGE - (reply: 3)
433. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
434. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
435. Help...purify and concentrate protein for IEF - (reply: 1)
436. preference of N/C terminus for tagging - (reply: 2)
437. NI-NTA purification - (reply: 2)
438. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
439. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
440. RNA immunoprecipitation...HELP! - (reply: 4)
441. Dialysis - A question about buffers (reply: 6)
442. Purification problems his-tag - I have tried everything (reply: 3)
443. GST-tagged protein binds to column but is not eluted - (reply: 1)
444. urea-denatured protein precipitates after dialysis - (reply: 1)
445. Expressing 4 proteins in an operon - (reply: 6)
446. More His-tag problems - (reply: 4)
447. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
448. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
449. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
450. Protein precipitates upon dialysis - (reply: 5)

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