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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Over Experssion of A Protein - (reply: 3)
422. IPTG induction not observable or noticeable - (reply: 2)
423. Problem with protein purification By FPLC - (reply: 4)
424. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
425. His-tag proteins not eluted from Ni-Beads - (reply: 2)
426. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
427. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
428. trouble with protein expression of high AT rich gene - (reply: 1)
429. Preparation of Purified Inclusion Bodies - (reply: 2)
430. Denaturing IB in 8 M Urea - (reply: 3)
431. Promega IVT Problems - Low expression (reply: 1)
432. weak bands - western blots (reply: 1)
433. problem in M9 media - m9 media (reply: 4)
434. Help for a purificaltion newbie - (reply: 2)
435. Immunioprecipitation from Jurkat cells?? - (reply: 1)
436. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
437. antibody HRP - (reply: 2)
438. Western blot transfer weak - (reply: 1)
439. Home-made Size-exclusion Columns? - (reply: 1)
440. how to get pure protein - (reply: 1)
441. co-Immunoprecipitation control question - co-IP (reply: 1)
442. to get protein in cytosol from inclusion bodies - (reply: 4)
443. Inclusion bodies in e.coli - (reply: 1)
444. lysis of e.coli by sonication - (reply: 2)
445. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
446. Protein expression - (reply: 8)
447. problem with ion exchange puification - protein not binding (reply: 3)
448. Protein Purification by precipitation - (reply: 2)
449. refolding proteins using dialysis - (reply: 1)
450. protein refolding - (reply: 1)

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