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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. lysis of e.coli by sonication - (reply: 2)
422. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
423. Protein expression - (reply: 8)
424. problem with ion exchange puification - protein not binding (reply: 3)
425. Protein Purification by precipitation - (reply: 2)
426. refolding proteins using dialysis - (reply: 1)
427. protein refolding - (reply: 1)
428. Zeocine resistence, pichia pastoris - (reply: 3)
429. Problem with MBP and GST tag purification - (reply: 1)
430. concentrating protein by method other than tff - (reply: 1)
431. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
432. M2 Flag purification - (reply: 2)
433. Quantitating IP'd proteins - (reply: 1)
434. Removal of Tightly Bound Nucleic Acids - (reply: 3)
435. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
436. Recombinant protein aggregation after purification - (reply: 2)
437. average total protein content per 293t cell - (reply: 2)
438. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
439. expression of MBP fusion protein - leaky and very low yield (reply: 2)
440. Calmodulin binding assay - (reply: 1)
441. strange problem with His purification - (reply: 1)
442. E.coli expression - (reply: 5)
443. why my protein precipitates? - (reply: 2)
444. DTT in GST elution buffer - (reply: 1)
445. vectors and his-tags - (reply: 3)
446. SDS boiled samples - (reply: 1)
447. E coli lysis by lysozyme - (reply: 2)
448. His-tag protein expression - (reply: 1)
449. SDS-PAGE loading dye - (reply: 1)
450. How to concentrate the protein - (reply: 4)

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