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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. M2 Flag purification - (reply: 2)
422. Quantitating IP'd proteins - (reply: 1)
423. Removal of Tightly Bound Nucleic Acids - (reply: 3)
424. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
425. Recombinant protein aggregation after purification - (reply: 2)
426. average total protein content per 293t cell - (reply: 2)
427. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
428. expression of MBP fusion protein - leaky and very low yield (reply: 2)
429. Calmodulin binding assay - (reply: 1)
430. strange problem with His purification - (reply: 1)
431. E.coli expression - (reply: 5)
432. why my protein precipitates? - (reply: 2)
433. DTT in GST elution buffer - (reply: 1)
434. vectors and his-tags - (reply: 3)
435. SDS boiled samples - (reply: 1)
436. E coli lysis by lysozyme - (reply: 2)
437. His-tag protein expression - (reply: 1)
438. SDS-PAGE loading dye - (reply: 1)
439. How to concentrate the protein - (reply: 4)
440. protein overexpression - factors affecting overexpression (reply: 3)
441. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
442. e coli strain selection for protein expression - (reply: 2)
443. Problem clariflying/filtering insect cell lysate - (reply: 2)
444. Ni column changing colors - (reply: 2)
445. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
446. Problem with Histag purification - (reply: 1)
447. Freezing eluted His tag protein - (reply: 5)
448. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
449. His-tag protein lost it's tag - (reply: 2)
450. AnionExchange problem: proteins do not bind - (reply: 12)

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