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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. protein overexpression - factors affecting overexpression (reply: 3)
422. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
423. e coli strain selection for protein expression - (reply: 2)
424. Problem clariflying/filtering insect cell lysate - (reply: 2)
425. Ni column changing colors - (reply: 2)
426. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
427. Problem with Histag purification - (reply: 1)
428. Freezing eluted His tag protein - (reply: 5)
429. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
430. His-tag protein lost it's tag - (reply: 2)
431. AnionExchange problem: proteins do not bind - (reply: 12)
432. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
433. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
434. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
435. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
436. IMPACT system purification problem - (reply: 1)
437. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
438. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
439. How to normalize my samples before I transfect my cells - (reply: 6)
440. FBS interference in SDS PAGE - (reply: 3)
441. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
442. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
443. Help...purify and concentrate protein for IEF - (reply: 1)
444. preference of N/C terminus for tagging - (reply: 2)
445. NI-NTA purification - (reply: 2)
446. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
447. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
448. RNA immunoprecipitation...HELP! - (reply: 4)
449. Dialysis - A question about buffers (reply: 6)
450. Purification problems his-tag - I have tried everything (reply: 3)

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