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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
422. His-tag proteins not eluted from Ni-Beads - (reply: 2)
423. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
424. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
425. trouble with protein expression of high AT rich gene - (reply: 1)
426. Preparation of Purified Inclusion Bodies - (reply: 2)
427. Denaturing IB in 8 M Urea - (reply: 3)
428. Promega IVT Problems - Low expression (reply: 1)
429. weak bands - western blots (reply: 1)
430. problem in M9 media - m9 media (reply: 4)
431. Help for a purificaltion newbie - (reply: 2)
432. Immunioprecipitation from Jurkat cells?? - (reply: 1)
433. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
434. antibody HRP - (reply: 2)
435. Western blot transfer weak - (reply: 1)
436. Home-made Size-exclusion Columns? - (reply: 1)
437. how to get pure protein - (reply: 1)
438. co-Immunoprecipitation control question - co-IP (reply: 1)
439. to get protein in cytosol from inclusion bodies - (reply: 4)
440. Inclusion bodies in e.coli - (reply: 1)
441. lysis of e.coli by sonication - (reply: 2)
442. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
443. Protein expression - (reply: 8)
444. problem with ion exchange puification - protein not binding (reply: 3)
445. Protein Purification by precipitation - (reply: 2)
446. refolding proteins using dialysis - (reply: 1)
447. protein refolding - (reply: 1)
448. Zeocine resistence, pichia pastoris - (reply: 3)
449. Problem with MBP and GST tag purification - (reply: 1)
450. concentrating protein by method other than tff - (reply: 1)

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