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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Protein expression - (reply: 8)
422. problem with ion exchange puification - protein not binding (reply: 3)
423. Protein Purification by precipitation - (reply: 2)
424. refolding proteins using dialysis - (reply: 1)
425. protein refolding - (reply: 1)
426. Zeocine resistence, pichia pastoris - (reply: 3)
427. Problem with MBP and GST tag purification - (reply: 1)
428. concentrating protein by method other than tff - (reply: 1)
429. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
430. M2 Flag purification - (reply: 2)
431. Quantitating IP'd proteins - (reply: 1)
432. Removal of Tightly Bound Nucleic Acids - (reply: 3)
433. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
434. Recombinant protein aggregation after purification - (reply: 2)
435. average total protein content per 293t cell - (reply: 2)
436. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
437. expression of MBP fusion protein - leaky and very low yield (reply: 2)
438. Calmodulin binding assay - (reply: 1)
439. strange problem with His purification - (reply: 1)
440. E.coli expression - (reply: 5)
441. why my protein precipitates? - (reply: 2)
442. DTT in GST elution buffer - (reply: 1)
443. vectors and his-tags - (reply: 3)
444. SDS boiled samples - (reply: 1)
445. E coli lysis by lysozyme - (reply: 2)
446. His-tag protein expression - (reply: 1)
447. SDS-PAGE loading dye - (reply: 1)
448. How to concentrate the protein - (reply: 4)
449. protein overexpression - factors affecting overexpression (reply: 3)
450. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)

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