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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
422. Help...purify and concentrate protein for IEF - (reply: 1)
423. preference of N/C terminus for tagging - (reply: 2)
424. NI-NTA purification - (reply: 2)
425. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
426. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
427. RNA immunoprecipitation...HELP! - (reply: 4)
428. Dialysis - A question about buffers (reply: 6)
429. Purification problems his-tag - I have tried everything (reply: 3)
430. GST-tagged protein binds to column but is not eluted - (reply: 1)
431. urea-denatured protein precipitates after dialysis - (reply: 1)
432. Expressing 4 proteins in an operon - (reply: 6)
433. More His-tag problems - (reply: 4)
434. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
435. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
436. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
437. Protein precipitates upon dialysis - (reply: 5)
438. membrane protein aggregation - (reply: 1)
439. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
440. His Tag Protein Purification - (reply: 6)
441. Amylose bound protein elution - (reply: 1)
442. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
443. Soluble aggregates - (reply: 3)
444. insect protein expression - (reply: 1)
445. Can you store TNT translation products? - (reply: 1)
446. How to pack the FPLC column - (reply: 2)
447. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
448. Sonication issues! - (reply: 3)
449. complete gene for expression? - (reply: 2)
450. buffer for membrane protein - (reply: 1)

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