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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. His-tag proteins not eluted from Ni-Beads - (reply: 2)
422. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
423. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
424. trouble with protein expression of high AT rich gene - (reply: 1)
425. Preparation of Purified Inclusion Bodies - (reply: 2)
426. Denaturing IB in 8 M Urea - (reply: 3)
427. Promega IVT Problems - Low expression (reply: 1)
428. weak bands - western blots (reply: 1)
429. problem in M9 media - m9 media (reply: 4)
430. Help for a purificaltion newbie - (reply: 2)
431. Immunioprecipitation from Jurkat cells?? - (reply: 1)
432. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
433. antibody HRP - (reply: 2)
434. Western blot transfer weak - (reply: 1)
435. Home-made Size-exclusion Columns? - (reply: 1)
436. how to get pure protein - (reply: 1)
437. co-Immunoprecipitation control question - co-IP (reply: 1)
438. to get protein in cytosol from inclusion bodies - (reply: 4)
439. Inclusion bodies in e.coli - (reply: 1)
440. lysis of e.coli by sonication - (reply: 2)
441. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
442. Protein expression - (reply: 8)
443. problem with ion exchange puification - protein not binding (reply: 3)
444. Protein Purification by precipitation - (reply: 2)
445. refolding proteins using dialysis - (reply: 1)
446. protein refolding - (reply: 1)
447. Zeocine resistence, pichia pastoris - (reply: 3)
448. Problem with MBP and GST tag purification - (reply: 1)
449. concentrating protein by method other than tff - (reply: 1)
450. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)

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