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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
422. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
423. trouble with protein expression of high AT rich gene - (reply: 1)
424. Preparation of Purified Inclusion Bodies - (reply: 2)
425. Denaturing IB in 8 M Urea - (reply: 3)
426. Promega IVT Problems - Low expression (reply: 1)
427. weak bands - western blots (reply: 1)
428. problem in M9 media - m9 media (reply: 4)
429. Help for a purificaltion newbie - (reply: 2)
430. Immunioprecipitation from Jurkat cells?? - (reply: 1)
431. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
432. antibody HRP - (reply: 2)
433. Western blot transfer weak - (reply: 1)
434. Home-made Size-exclusion Columns? - (reply: 1)
435. how to get pure protein - (reply: 1)
436. co-Immunoprecipitation control question - co-IP (reply: 1)
437. to get protein in cytosol from inclusion bodies - (reply: 4)
438. Inclusion bodies in e.coli - (reply: 1)
439. lysis of e.coli by sonication - (reply: 2)
440. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
441. Protein expression - (reply: 8)
442. problem with ion exchange puification - protein not binding (reply: 3)
443. Protein Purification by precipitation - (reply: 2)
444. refolding proteins using dialysis - (reply: 1)
445. protein refolding - (reply: 1)
446. Zeocine resistence, pichia pastoris - (reply: 3)
447. Problem with MBP and GST tag purification - (reply: 1)
448. concentrating protein by method other than tff - (reply: 1)
449. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
450. M2 Flag purification - (reply: 2)

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