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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
421. contamination in protein expression - I got a couple of questions (reply: 3)
422. Always expressing truncated protein - (reply: 3)
423. Over Experssion of A Protein - (reply: 3)
424. IPTG induction not observable or noticeable - (reply: 2)
425. Problem with protein purification By FPLC - (reply: 4)
426. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
427. His-tag proteins not eluted from Ni-Beads - (reply: 2)
428. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
429. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
430. trouble with protein expression of high AT rich gene - (reply: 1)
431. Preparation of Purified Inclusion Bodies - (reply: 2)
432. Denaturing IB in 8 M Urea - (reply: 3)
433. Promega IVT Problems - Low expression (reply: 1)
434. weak bands - western blots (reply: 1)
435. problem in M9 media - m9 media (reply: 4)
436. Help for a purificaltion newbie - (reply: 2)
437. Immunioprecipitation from Jurkat cells?? - (reply: 1)
438. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
439. antibody HRP - (reply: 2)
440. Western blot transfer weak - (reply: 1)
441. Home-made Size-exclusion Columns? - (reply: 1)
442. how to get pure protein - (reply: 1)
443. co-Immunoprecipitation control question - co-IP (reply: 1)
444. to get protein in cytosol from inclusion bodies - (reply: 4)
445. Inclusion bodies in e.coli - (reply: 1)
446. lysis of e.coli by sonication - (reply: 2)
447. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
448. Protein expression - (reply: 8)
449. problem with ion exchange puification - protein not binding (reply: 3)
450. Protein Purification by precipitation - (reply: 2)

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