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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. EMSA - general question - (reply: 1)
182. Understanding immunoprecipitation - where do the beads go? - (reply: 2)
183. Soluble protien of GST - (reply: 2)
184. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
185. Contaminants in protein purification.....help! - (reply: 3)
186. Protein expression and purification - Please help - (reply: 2)
187. Protein purifcation without chromatography - (reply: 6)
188. Bacterial Media Formulation - (reply: 2)
189. tag for protein purification - (reply: 7)
190. Protein is not Binding to DE52 column material - (reply: 2)
191. expression problem with pBAD33...similar experience? - (reply: 2)
192. MBP-Tagged protein degrading after expression - (reply: 1)
193. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
194. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
195. Controls for co-immunoprecipitation - (reply: 3)
196. How to improve enzyme activity? - (reply: 2)
197. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
198. Sediments/debris/pellet in protein lysate? - (reply: 3)
199. Cleavage of GST-tag with Factor Xa - (reply: 1)
200. Trouble in GST- fusion protein purification - (reply: 1)
201. Helppurification of expressed protein - (reply: 2)
202. protein expression by changing start codon - (reply: 1)
203. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
204. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
205. pcDNA overexpression - (reply: 4)
206. membrane proteins ultrafiltration - (reply: 2)
207. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
208. Interferon Alpha - (reply: 1)
209. Can you see GFP after lysis mammalian cells? - (reply: 5)
210. Very small peptide expression in E. coli - (reply: 2)

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