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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. brain tissue homogenization with sample buffer?? - (reply: 2)
182. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
183. Reversed-Phase chromatography for protein purification - (reply: 3)
184. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
185. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
186. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
187. need help with gst-tagged protein-purification - (reply: 1)
188. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
189. Protein degradation during expression - (reply: 3)
190. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
191. Expression of insoluble protein - (reply: 4)
192. Weak affinity chromatography - (reply: 2)
193. NL and L? - (reply: 2)
194. e coli vector and host selection - (reply: 1)
195. Double tagging - (reply: 1)
196. Purifying recombinant protein from pellet? - (reply: 2)
197. Co-expression of 2 plasmids in E.coli - (reply: 1)
198. Protein ladder - (reply: 1)
199. Trouble producing stable cell lines - (reply: 2)
200. Can anyone help me to identify what line is that??? - (reply: 4)
201. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
202. membrane protein expression - (reply: 1)
203. Magnesium acetate in protein lysates - (reply: 1)
204. Protease Inhibitor Cocktails - (reply: 3)
205. E. coli cells refuse to grow! - (reply: 2)
206. Extra bands after protein expression/purification - (reply: 4)
207. Better protein Expression? - (reply: 2)
208. Expression of protein with pI 8.2 - (reply: 1)
209. Methionine for protein expression - (reply: 3)
210. Alternatives to benzonase? - (reply: 1)

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