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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
182. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
183. Controls for co-immunoprecipitation - (reply: 3)
184. How to improve enzyme activity? - (reply: 2)
185. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
186. Sediments/debris/pellet in protein lysate? - (reply: 3)
187. Cleavage of GST-tag with Factor Xa - (reply: 1)
188. Trouble in GST- fusion protein purification - (reply: 1)
189. Helppurification of expressed protein - (reply: 2)
190. protein expression by changing start codon - (reply: 1)
191. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
192. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
193. pcDNA overexpression - (reply: 4)
194. membrane proteins ultrafiltration - (reply: 2)
195. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
196. Interferon Alpha - (reply: 1)
197. Can you see GFP after lysis mammalian cells? - (reply: 5)
198. Very small peptide expression in E. coli - (reply: 2)
199. C-terminal GST fusion problem in mammalian cell - (reply: 6)
200. Is anti-His antibody detergent sensitive? - (reply: 6)
201. tagged protein size - (reply: 10)
202. Protein expression in e.coli - (reply: 5)
203. Problems with overnights - (reply: 5)
204. problem in extracting the protein - (reply: 1)
205. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
206. Protein Purification from Polyacrylamide Gels - (reply: 3)
207. Protein Expression vector with small tag - (reply: 1)
208. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
209. Albumin Removal From BSA - (reply: 1)
210. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)

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