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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
182. Contaminants in protein purification.....help! - (reply: 3)
183. Protein expression and purification - Please help - (reply: 2)
184. Protein purifcation without chromatography - (reply: 6)
185. Bacterial Media Formulation - (reply: 2)
186. tag for protein purification - (reply: 7)
187. Protein is not Binding to DE52 column material - (reply: 2)
188. expression problem with pBAD33...similar experience? - (reply: 2)
189. MBP-Tagged protein degrading after expression - (reply: 1)
190. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
191. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
192. Controls for co-immunoprecipitation - (reply: 3)
193. How to improve enzyme activity? - (reply: 2)
194. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
195. Sediments/debris/pellet in protein lysate? - (reply: 3)
196. Cleavage of GST-tag with Factor Xa - (reply: 1)
197. Trouble in GST- fusion protein purification - (reply: 1)
198. Helppurification of expressed protein - (reply: 2)
199. protein expression by changing start codon - (reply: 1)
200. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
201. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
202. pcDNA overexpression - (reply: 4)
203. membrane proteins ultrafiltration - (reply: 2)
204. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
205. Interferon Alpha - (reply: 1)
206. Can you see GFP after lysis mammalian cells? - (reply: 5)
207. Very small peptide expression in E. coli - (reply: 2)
208. C-terminal GST fusion problem in mammalian cell - (reply: 6)
209. Is anti-His antibody detergent sensitive? - (reply: 6)
210. tagged protein size - (reply: 10)

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