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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
182. Multiple protein bands after expression - (reply: 6)
183. his-tag prufication lysis problem:( - (reply: 4)
184. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
185. co-eluted protein with his-tag protein - (reply: 1)
186. Help with His-Tag protein purification - (reply: 2)
187. Discrepancy between tissue and cell culture - (reply: 1)
188. Expression and Lysozyme - (reply: 3)
189. Rosetta-gami B Incubation Time - (reply: 1)
190. Decreased protein yields after successive passaging? - (reply: 1)
191. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
192. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
193. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
194. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
195. how much time for prepared gel to keep bfore loading? - (reply: 5)
196. plant extraction procedure for protein - (reply: 3)
197. brain tissue homogenization with sample buffer?? - (reply: 2)
198. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
199. Reversed-Phase chromatography for protein purification - (reply: 3)
200. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
201. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
202. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
203. need help with gst-tagged protein-purification - (reply: 1)
204. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
205. Protein degradation during expression - (reply: 3)
206. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
207. Expression of insoluble protein - (reply: 4)
208. Weak affinity chromatography - (reply: 2)
209. NL and L? - (reply: 2)
210. e coli vector and host selection - (reply: 1)

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