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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. pcDNA overexpression - (reply: 4)
182. membrane proteins ultrafiltration - (reply: 2)
183. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
184. Interferon Alpha - (reply: 1)
185. Can you see GFP after lysis mammalian cells? - (reply: 5)
186. Very small peptide expression in E. coli - (reply: 2)
187. C-terminal GST fusion problem in mammalian cell - (reply: 6)
188. Is anti-His antibody detergent sensitive? - (reply: 6)
189. tagged protein size - (reply: 10)
190. Protein expression in e.coli - (reply: 5)
191. Problems with overnights - (reply: 5)
192. problem in extracting the protein - (reply: 1)
193. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
194. Protein Purification from Polyacrylamide Gels - (reply: 3)
195. Protein Expression vector with small tag - (reply: 1)
196. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
197. Albumin Removal From BSA - (reply: 1)
198. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
199. Insulin production - (reply: 2)
200. High Yield Protein Expression 10g/L - (reply: 5)
201. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
202. GST- tagged protein expressed in insoluble - (reply: 5)
203. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
204. Prepare protein standards for concentration determination - (reply: 1)
205. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
206. Tagged recombinant protein has different mobility? - (reply: 4)
207. My protein doesn't bind to the Ni-NTA column - (reply: 11)
208. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
209. TCA IgG precipitation - (reply: 2)
210. Does triple labeled protein precipitate more easily? - (reply: 2)

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