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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. protein expression by changing start codon - (reply: 1)
182. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
183. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
184. pcDNA overexpression - (reply: 4)
185. membrane proteins ultrafiltration - (reply: 2)
186. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
187. Interferon Alpha - (reply: 1)
188. Can you see GFP after lysis mammalian cells? - (reply: 5)
189. Very small peptide expression in E. coli - (reply: 2)
190. C-terminal GST fusion problem in mammalian cell - (reply: 6)
191. Is anti-His antibody detergent sensitive? - (reply: 6)
192. tagged protein size - (reply: 10)
193. Protein expression in e.coli - (reply: 5)
194. Problems with overnights - (reply: 5)
195. problem in extracting the protein - (reply: 1)
196. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
197. Protein Purification from Polyacrylamide Gels - (reply: 3)
198. Protein Expression vector with small tag - (reply: 1)
199. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
200. Albumin Removal From BSA - (reply: 1)
201. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
202. Insulin production - (reply: 2)
203. High Yield Protein Expression 10g/L - (reply: 5)
204. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
205. GST- tagged protein expressed in insoluble - (reply: 5)
206. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
207. Prepare protein standards for concentration determination - (reply: 1)
208. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
209. Tagged recombinant protein has different mobility? - (reply: 6)
210. My protein doesn't bind to the Ni-NTA column - (reply: 11)

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