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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Can you see GFP after lysis mammalian cells? - (reply: 5)
182. Very small peptide expression in E. coli - (reply: 2)
183. C-terminal GST fusion problem in mammalian cell - (reply: 6)
184. Is anti-His antibody detergent sensitive? - (reply: 6)
185. tagged protein size - (reply: 10)
186. Protein expression in e.coli - (reply: 5)
187. Problems with overnights - (reply: 5)
188. problem in extracting the protein - (reply: 1)
189. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
190. Protein Purification from Polyacrylamide Gels - (reply: 3)
191. Protein Expression vector with small tag - (reply: 1)
192. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
193. Albumin Removal From BSA - (reply: 1)
194. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
195. Insulin production - (reply: 2)
196. High Yield Protein Expression 10g/L - (reply: 5)
197. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
198. GST- tagged protein expressed in insoluble - (reply: 5)
199. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
200. Prepare protein standards for concentration determination - (reply: 1)
201. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
202. Tagged recombinant protein has different mobility? - (reply: 4)
203. My protein doesn't bind to the Ni-NTA column - (reply: 11)
204. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
205. TCA IgG precipitation - (reply: 2)
206. Does triple labeled protein precipitate more easily? - (reply: 2)
207. High gravity crush beads ? - (reply: 1)
208. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
209. how do GST-tags affect gel filtration results? - (reply: 5)
210. protein extraction from human tissue (very small) to do ELISA - (reply: 5)

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