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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Protein expression and purification - Please help - (reply: 2)
182. Protein purifcation without chromatography - (reply: 6)
183. Bacterial Media Formulation - (reply: 2)
184. tag for protein purification - (reply: 7)
185. Protein is not Binding to DE52 column material - (reply: 2)
186. expression problem with pBAD33...similar experience? - (reply: 2)
187. MBP-Tagged protein degrading after expression - (reply: 1)
188. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
189. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
190. Controls for co-immunoprecipitation - (reply: 3)
191. How to improve enzyme activity? - (reply: 2)
192. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
193. Sediments/debris/pellet in protein lysate? - (reply: 3)
194. Cleavage of GST-tag with Factor Xa - (reply: 1)
195. Trouble in GST- fusion protein purification - (reply: 1)
196. Helppurification of expressed protein - (reply: 2)
197. protein expression by changing start codon - (reply: 1)
198. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
199. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
200. pcDNA overexpression - (reply: 4)
201. membrane proteins ultrafiltration - (reply: 2)
202. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
203. Interferon Alpha - (reply: 1)
204. Can you see GFP after lysis mammalian cells? - (reply: 5)
205. Very small peptide expression in E. coli - (reply: 2)
206. C-terminal GST fusion problem in mammalian cell - (reply: 6)
207. Is anti-His antibody detergent sensitive? - (reply: 6)
208. tagged protein size - (reply: 10)
209. Protein expression in e.coli - (reply: 5)
210. Problems with overnights - (reply: 5)

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