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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Protein is not Binding to DE52 column material - (reply: 2)
182. expression problem with pBAD33...similar experience? - (reply: 2)
183. MBP-Tagged protein degrading after expression - (reply: 1)
184. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
185. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
186. Controls for co-immunoprecipitation - (reply: 3)
187. How to improve enzyme activity? - (reply: 2)
188. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
189. Sediments/debris/pellet in protein lysate? - (reply: 3)
190. Cleavage of GST-tag with Factor Xa - (reply: 1)
191. Trouble in GST- fusion protein purification - (reply: 1)
192. Helppurification of expressed protein - (reply: 2)
193. protein expression by changing start codon - (reply: 1)
194. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
195. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
196. pcDNA overexpression - (reply: 4)
197. membrane proteins ultrafiltration - (reply: 2)
198. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
199. Interferon Alpha - (reply: 1)
200. Can you see GFP after lysis mammalian cells? - (reply: 5)
201. Very small peptide expression in E. coli - (reply: 2)
202. C-terminal GST fusion problem in mammalian cell - (reply: 6)
203. Is anti-His antibody detergent sensitive? - (reply: 6)
204. tagged protein size - (reply: 10)
205. Protein expression in e.coli - (reply: 5)
206. Problems with overnights - (reply: 5)
207. problem in extracting the protein - (reply: 1)
208. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
209. Protein Purification from Polyacrylamide Gels - (reply: 3)
210. Protein Expression vector with small tag - (reply: 1)

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