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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. tag for protein purification - (reply: 7)
182. Protein is not Binding to DE52 column material - (reply: 2)
183. expression problem with pBAD33...similar experience? - (reply: 2)
184. MBP-Tagged protein degrading after expression - (reply: 1)
185. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
186. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
187. Controls for co-immunoprecipitation - (reply: 3)
188. How to improve enzyme activity? - (reply: 2)
189. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
190. Sediments/debris/pellet in protein lysate? - (reply: 3)
191. Cleavage of GST-tag with Factor Xa - (reply: 1)
192. Trouble in GST- fusion protein purification - (reply: 1)
193. Helppurification of expressed protein - (reply: 2)
194. protein expression by changing start codon - (reply: 1)
195. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
196. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
197. pcDNA overexpression - (reply: 4)
198. membrane proteins ultrafiltration - (reply: 2)
199. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
200. Interferon Alpha - (reply: 1)
201. Can you see GFP after lysis mammalian cells? - (reply: 5)
202. Very small peptide expression in E. coli - (reply: 2)
203. C-terminal GST fusion problem in mammalian cell - (reply: 6)
204. Is anti-His antibody detergent sensitive? - (reply: 6)
205. tagged protein size - (reply: 10)
206. Protein expression in e.coli - (reply: 5)
207. Problems with overnights - (reply: 5)
208. problem in extracting the protein - (reply: 1)
209. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
210. Protein Purification from Polyacrylamide Gels - (reply: 3)

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