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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Trouble in GST- fusion protein purification - (reply: 1)
182. Helppurification of expressed protein - (reply: 2)
183. protein expression by changing start codon - (reply: 1)
184. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
185. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
186. pcDNA overexpression - (reply: 4)
187. membrane proteins ultrafiltration - (reply: 2)
188. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
189. Interferon Alpha - (reply: 1)
190. Can you see GFP after lysis mammalian cells? - (reply: 5)
191. Very small peptide expression in E. coli - (reply: 2)
192. C-terminal GST fusion problem in mammalian cell - (reply: 6)
193. Is anti-His antibody detergent sensitive? - (reply: 6)
194. tagged protein size - (reply: 10)
195. Protein expression in e.coli - (reply: 5)
196. Problems with overnights - (reply: 5)
197. problem in extracting the protein - (reply: 1)
198. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
199. Protein Purification from Polyacrylamide Gels - (reply: 3)
200. Protein Expression vector with small tag - (reply: 1)
201. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
202. Albumin Removal From BSA - (reply: 1)
203. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
204. Insulin production - (reply: 2)
205. High Yield Protein Expression 10g/L - (reply: 5)
206. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
207. GST- tagged protein expressed in insoluble - (reply: 5)
208. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
209. Prepare protein standards for concentration determination - (reply: 1)
210. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)

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