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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. C-terminal GST fusion problem in mammalian cell - (reply: 6)
182. Is anti-His antibody detergent sensitive? - (reply: 6)
183. tagged protein size - (reply: 10)
184. Protein expression in e.coli - (reply: 5)
185. Problems with overnights - (reply: 5)
186. problem in extracting the protein - (reply: 1)
187. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
188. Protein Purification from Polyacrylamide Gels - (reply: 3)
189. Protein Expression vector with small tag - (reply: 1)
190. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
191. Albumin Removal From BSA - (reply: 1)
192. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
193. Insulin production - (reply: 2)
194. High Yield Protein Expression 10g/L - (reply: 5)
195. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
196. GST- tagged protein expressed in insoluble - (reply: 5)
197. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
198. Prepare protein standards for concentration determination - (reply: 1)
199. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
200. Tagged recombinant protein has different mobility? - (reply: 4)
201. My protein doesn't bind to the Ni-NTA column - (reply: 11)
202. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
203. TCA IgG precipitation - (reply: 2)
204. Does triple labeled protein precipitate more easily? - (reply: 2)
205. High gravity crush beads ? - (reply: 1)
206. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
207. how do GST-tags affect gel filtration results? - (reply: 5)
208. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
209. Stable protein expression in Vero cells - (reply: 2)
210. Which kind of ATP do you usually use in you experiment? - (reply: 2)

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