Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. expression problem with pBAD33...similar experience? - (reply: 2)
182. MBP-Tagged protein degrading after expression - (reply: 1)
183. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
184. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
185. Controls for co-immunoprecipitation - (reply: 3)
186. How to improve enzyme activity? - (reply: 2)
187. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
188. Sediments/debris/pellet in protein lysate? - (reply: 3)
189. Cleavage of GST-tag with Factor Xa - (reply: 1)
190. Trouble in GST- fusion protein purification - (reply: 1)
191. Helppurification of expressed protein - (reply: 2)
192. protein expression by changing start codon - (reply: 1)
193. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
194. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
195. pcDNA overexpression - (reply: 4)
196. membrane proteins ultrafiltration - (reply: 2)
197. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
198. Interferon Alpha - (reply: 1)
199. Can you see GFP after lysis mammalian cells? - (reply: 5)
200. Very small peptide expression in E. coli - (reply: 2)
201. C-terminal GST fusion problem in mammalian cell - (reply: 6)
202. Is anti-His antibody detergent sensitive? - (reply: 6)
203. tagged protein size - (reply: 10)
204. Protein expression in e.coli - (reply: 5)
205. Problems with overnights - (reply: 5)
206. problem in extracting the protein - (reply: 1)
207. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
208. Protein Purification from Polyacrylamide Gels - (reply: 3)
209. Protein Expression vector with small tag - (reply: 1)
210. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22