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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
181. Cleavage of GST-tag with Factor Xa - (reply: 1)
182. Trouble in GST- fusion protein purification - (reply: 1)
183. Helppurification of expressed protein - (reply: 2)
184. protein expression by changing start codon - (reply: 1)
185. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
186. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
187. pcDNA overexpression - (reply: 4)
188. membrane proteins ultrafiltration - (reply: 2)
189. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
190. Interferon Alpha - (reply: 1)
191. Can you see GFP after lysis mammalian cells? - (reply: 5)
192. Very small peptide expression in E. coli - (reply: 2)
193. C-terminal GST fusion problem in mammalian cell - (reply: 6)
194. Is anti-His antibody detergent sensitive? - (reply: 6)
195. tagged protein size - (reply: 10)
196. Protein expression in e.coli - (reply: 5)
197. Problems with overnights - (reply: 5)
198. problem in extracting the protein - (reply: 1)
199. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
200. Protein Purification from Polyacrylamide Gels - (reply: 3)
201. Protein Expression vector with small tag - (reply: 1)
202. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
203. Albumin Removal From BSA - (reply: 1)
204. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
205. Insulin production - (reply: 2)
206. High Yield Protein Expression 10g/L - (reply: 5)
207. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
208. GST- tagged protein expressed in insoluble - (reply: 5)
209. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
210. Prepare protein standards for concentration determination - (reply: 1)

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