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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Best epitope for IF with a poor-expresser - (reply: 2)
2. Collagen binding protein expression E. coli His-tag - (reply: 1)
3. pet-32 - (reply: 2)
4. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
5. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
6. unexpected protein bands - (reply: 2)
7. Urea effect on mice spleen cells - (reply: 1)
8. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
9. Validating Recombinant Protein - (reply: 1)
10. Sonication of cells in ultrasound bath, without probe - (reply: 3)
11. Proteinase extraction and purification - (reply: 1)
12. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
13. EGFP expression - (reply: 1)
14. the same construct, two stable cell clones with different protein size - (reply: 2)
15. Purification of metallothioneins? - (reply: 1)
16. RIPA buffer for IP and western - (reply: 1)
17. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
18. CO- IP for nuclear protein - (reply: 4)
19. Trichloroacetic Acid precipitation of proteins - (reply: 2)
20. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
21. protein expression with and without induction - (reply: 1)
22. Please help me :Protein expression and purification - (reply: 1)
23. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
24. genotype of e coli strain for protein expression - (reply: 2)
25. Problems with detection of protein expressed in yeast - (reply: 1)
26. thionin - (reply: 1)
27. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
28. secretome and zymograms - (reply: 1)
29. Protein Refolding - Precipitation during dialysis - (reply: 3)
30. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)

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