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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
2. protein expression site - (reply: 3)
3. Beads pull down only free GST - (reply: 14)
4. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
5. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
6. protein expression - (reply: 2)
7. Anion Exchange Buffers - (reply: 2)
8. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
9. about sds-page buffers - (reply: 1)
10. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
11. Best epitope for IF with a poor-expresser - (reply: 2)
12. Collagen binding protein expression E. coli His-tag - (reply: 1)
13. pet-32 - (reply: 3)
14. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
15. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
16. Question about MBP purification - (reply: 2)
17. unexpected protein bands - (reply: 2)
18. Urea effect on mice spleen cells - (reply: 1)
19. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
20. Validating Recombinant Protein - (reply: 1)
21. Sonication of cells in ultrasound bath, without probe - (reply: 3)
22. Proteinase extraction and purification - (reply: 1)
23. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
24. EGFP expression - (reply: 1)
25. the same construct, two stable cell clones with different protein size - (reply: 2)
26. Purification of metallothioneins? - (reply: 1)
27. RIPA buffer for IP and western - (reply: 1)
28. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
29. CO- IP for nuclear protein - (reply: 4)
30. Trichloroacetic Acid precipitation of proteins - (reply: 2)

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