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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Validating Recombinant Protein - (reply: 1)
2. Sonication of cells in ultrasound bath, without probe - (reply: 3)
3. Proteinase extraction and purification - (reply: 1)
4. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
5. EGFP expression - (reply: 1)
6. the same construct, two stable cell clones with different protein size - (reply: 2)
7. Purification of metallothioneins? - (reply: 1)
8. RIPA buffer for IP and western - (reply: 1)
9. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
10. CO- IP for nuclear protein - (reply: 4)
11. Trichloroacetic Acid precipitation of proteins - (reply: 2)
12. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
13. protein expression with and without induction - (reply: 1)
14. Please help me :Protein expression and purification - (reply: 1)
15. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
16. genotype of e coli strain for protein expression - (reply: 2)
17. Problems with detection of protein expressed in yeast - (reply: 1)
18. thionin - (reply: 1)
19. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
20. secretome and zymograms - (reply: 1)
21. Protein Refolding - Precipitation during dialysis - (reply: 3)
22. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
23. Problem with calculations for enzyme activity - (reply: 2)
24. problem with sds-page - (reply: 8)
25. Ammonium sulfate fractionalization help - (reply: 1)
26. heme protein precipitation - (reply: 4)
27. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 3)
28. regarding protein purification using 8m urea and sds page - (reply: 2)
29. how do you inactivate His-tag affinity beads?? - (reply: 4)
30. Protein Purification from mammalian cells - (reply: 1)

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