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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Trichloroacetic Acid precipitation of proteins - (reply: 1)
2. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 2)
3. protein expression with and without induction - (reply: 1)
4. Please help me :Protein expression and purification - (reply: 1)
5. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
6. genotype of e coli strain for protein expression - (reply: 2)
7. Problems with detection of protein expressed in yeast - (reply: 1)
8. thionin - (reply: 1)
9. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
10. secretome and zymograms - (reply: 1)
11. Protein Refolding - Precipitation during dialysis - (reply: 3)
12. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
13. Problem with calculations for enzyme activity - (reply: 2)
14. problem with sds-page - (reply: 8)
15. Ammonium sulfate fractionalization help - (reply: 1)
16. heme protein precipitation - (reply: 4)
17. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 3)
18. regarding protein purification using 8m urea and sds page - (reply: 2)
19. how do you inactivate His-tag affinity beads?? - (reply: 4)
20. Protein Purification from mammalian cells - (reply: 1)
21. DNA cleanup after linearization, before transformation? - (reply: 3)
22. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
23. predict VLP formation - (reply: 1)
24. need help for transfection to synchronized cells - (reply: 4)
25. Pulldown of polyubiquitinated proteins - (reply: 1)
26. membrane protein expression - (reply: 2)
27. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
28. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
29. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
30. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)

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