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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Pros and Cons of refolding proteins - (reply: 2)
2. pKD1 based vector for K. lactis expression - (reply: 2)
3. HSP70 contamination after Nickel purification - (reply: 3)
4. Use of urea in solubilizing protein and its aftermath - (reply: 5)
5. GST fusion protein - two dominant bands - (reply: 3)
6. Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
7. Increasing protein solubility in general - (reply: 3)
8. Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
9. Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
10. Doubt on HisTag protein purification miniprep - (reply: 3)
11. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
12. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
13. after gelfiltration need to concentrate peptide mix - (reply: 4)
14. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
15. Protein that used to express really well stopped expressing. - (reply: 2)
16. Determining Cofactor for Inactive Enzyme - (reply: 6)
17. study protein expression in bacteria - (reply: 1)
18. total protein measurement from insect body - (reply: 1)
19. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
20. Large-scale cell growth and protein expression - (reply: 1)
21. Reduce protein concentration without reduced enzyme activity - (reply: 3)
22. consistent expression of overlarge proteins - (reply: 1)
23. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
24. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
25. storage conditions for purified protein - (reply: 2)
26. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
27. Heat shock vs eletroporation - (reply: 6)
28. lysate protein concentration - (reply: 1)
29. Cloned gene in pET system not being expressed - (reply: 10)
30. recombinant protein expression- surface or internal? - (reply: 3)

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