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1. Desalting resin - (reply: 1)
2. RNA extraction by trizol - (reply: 3)
3. Problems in expression of target protein in E. coli - (reply: 2)
4. Membrane protein extraction - (reply: 2)
5. Is it possible to use wireless connection to control and monitor FPLC? - (reply: 2)
6. EMSA - general question - (reply: 1)
7. Understanding immunoprecipitation - where do the beads go? - (reply: 1)
8. Soluble protien of GST - (reply: 2)
9. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
10. Contaminants in protein purification.....help! - (reply: 3)
11. Protein expression and purification - Please help - (reply: 1)
12. Protein purifcation without chromatography - (reply: 6)
13. Bacterial Media Formulation - (reply: 2)
14. tag for protein purification - (reply: 4)
15. Protein is not Binding to DE52 column material - (reply: 2)
16. expression problem with pBAD33...similar experience? - (reply: 2)
17. MBP-Tagged protein degrading after expression - (reply: 1)
18. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
19. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
20. Controls for co-immunoprecipitation - (reply: 3)
21. How to improve enzyme activity? - (reply: 2)
22. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
23. Sediments/debris/pellet in protein lysate? - (reply: 3)
24. Cleavage of GST-tag with Factor Xa - (reply: 1)
25. Trouble in GST- fusion protein purification - (reply: 1)
26. Helppurification of expressed protein - (reply: 2)
27. protein expression by changing start codon - (reply: 1)
28. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
29. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
30. pcDNA overexpression - (reply: 4)
2. RNA extraction by trizol - (reply: 3)
3. Problems in expression of target protein in E. coli - (reply: 2)
4. Membrane protein extraction - (reply: 2)
5. Is it possible to use wireless connection to control and monitor FPLC? - (reply: 2)
6. EMSA - general question - (reply: 1)
7. Understanding immunoprecipitation - where do the beads go? - (reply: 1)
8. Soluble protien of GST - (reply: 2)
9. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
10. Contaminants in protein purification.....help! - (reply: 3)
11. Protein expression and purification - Please help - (reply: 1)
12. Protein purifcation without chromatography - (reply: 6)
13. Bacterial Media Formulation - (reply: 2)
14. tag for protein purification - (reply: 4)
15. Protein is not Binding to DE52 column material - (reply: 2)
16. expression problem with pBAD33...similar experience? - (reply: 2)
17. MBP-Tagged protein degrading after expression - (reply: 1)
18. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
19. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
20. Controls for co-immunoprecipitation - (reply: 3)
21. How to improve enzyme activity? - (reply: 2)
22. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
23. Sediments/debris/pellet in protein lysate? - (reply: 3)
24. Cleavage of GST-tag with Factor Xa - (reply: 1)
25. Trouble in GST- fusion protein purification - (reply: 1)
26. Helppurification of expressed protein - (reply: 2)
27. protein expression by changing start codon - (reply: 1)
28. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
29. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
30. pcDNA overexpression - (reply: 4)