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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. Protein localisation - IHC - (reply: 1)
2. Collagen Tipe I filtration - (reply: 1)
3. Removal of cleaved peptide after Protease cleavage - (reply: 4)
4. Bradford positive but no Protein on SDS PAGE - (reply: 3)
5. Disadvantage of crossflow filtration - (reply: 3)
6. Help with Superdex200 increase Column - (reply: 1)
7. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
8. Loss of protein expression by transformed bacteria - (reply: 3)
9. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
10. after protein purification - (reply: 4)
11. Tryple express vs scraping - (reply: 2)
12. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
13. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
14. recombinant protein vs native protein - (reply: 1)
15. protein expression site - (reply: 3)
16. Beads pull down only free GST - (reply: 14)
17. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
18. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
19. protein expression - (reply: 2)
20. Anion Exchange Buffers - (reply: 2)
21. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
22. about sds-page buffers - (reply: 1)
23. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
24. Best epitope for IF with a poor-expresser - (reply: 2)
25. Collagen binding protein expression E. coli His-tag - (reply: 1)
26. pet-32 - (reply: 3)
27. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
28. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
29. Question about MBP purification - (reply: 2)
30. unexpected protein bands - (reply: 2)

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