Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
1. His-tag protein not binding to resin - (reply: 8)
2. Protein localisation - IHC - (reply: 1)
3. Collagen Tipe I filtration - (reply: 1)
4. Removal of cleaved peptide after Protease cleavage - (reply: 4)
5. Bradford positive but no Protein on SDS PAGE - (reply: 3)
6. Disadvantage of crossflow filtration - (reply: 3)
7. Help with Superdex200 increase Column - (reply: 1)
8. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
9. Loss of protein expression by transformed bacteria - (reply: 4)
10. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
11. after protein purification - (reply: 4)
12. Tryple express vs scraping - (reply: 2)
13. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
14. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
15. recombinant protein vs native protein - (reply: 1)
16. protein expression site - (reply: 3)
17. Beads pull down only free GST - (reply: 14)
18. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
19. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
20. protein expression - (reply: 2)
21. Anion Exchange Buffers - (reply: 2)
22. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
23. about sds-page buffers - (reply: 1)
24. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
25. Best epitope for IF with a poor-expresser - (reply: 2)
26. Collagen binding protein expression E. coli His-tag - (reply: 1)
27. pet-32 - (reply: 3)
28. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
29. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
30. Question about MBP purification - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21