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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. how do you inactivate His-tag affinity beads?? - (reply: 4)
62. Protein Purification from mammalian cells - (reply: 1)
63. DNA cleanup after linearization, before transformation? - (reply: 3)
64. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
65. predict VLP formation - (reply: 1)
66. need help for transfection to synchronized cells - (reply: 4)
67. Pulldown of polyubiquitinated proteins - (reply: 1)
68. membrane protein expression - (reply: 2)
69. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
70. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
71. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
72. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
73. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
74. protein extraction from cell nucleus - (reply: 2)
75. his-tag purification - (reply: 9)
76. selection of the media for ion exchange chromatography - (reply: 1)
77. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
78. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
79. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
80. His-tag and GST-tag - (reply: 1)
81. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
82. Do protein purification under partially denatured condition - (reply: 1)
83. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
84. how to remove affinity tag completely - (reply: 14)
85. Agarose bead size in affinity chromatography - (reply: 2)
86. Protein precipitation by storage - (reply: 11)
87. designing fusion protein with Flag tag - (reply: 1)
88. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
89. Control protein for eukaryotic expression - (reply: 5)
90. Flag fusion protein not detected by Flag antibody - (reply: 8)

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