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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
62. EGFP expression - (reply: 1)
63. the same construct, two stable cell clones with different protein size - (reply: 2)
64. Purification of metallothioneins? - (reply: 1)
65. RIPA buffer for IP and western - (reply: 1)
66. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
67. CO- IP for nuclear protein - (reply: 4)
68. Trichloroacetic Acid precipitation of proteins - (reply: 2)
69. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
70. protein expression with and without induction - (reply: 1)
71. Please help me :Protein expression and purification - (reply: 1)
72. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
73. genotype of e coli strain for protein expression - (reply: 2)
74. Problems with detection of protein expressed in yeast - (reply: 1)
75. thionin - (reply: 1)
76. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
77. secretome and zymograms - (reply: 1)
78. Protein Refolding - Precipitation during dialysis - (reply: 3)
79. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
80. Problem with calculations for enzyme activity - (reply: 2)
81. problem with sds-page - (reply: 8)
82. Ammonium sulfate fractionalization help - (reply: 1)
83. heme protein precipitation - (reply: 4)
84. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
85. regarding protein purification using 8m urea and sds page - (reply: 2)
86. how do you inactivate His-tag affinity beads?? - (reply: 4)
87. Protein Purification from mammalian cells - (reply: 1)
88. DNA cleanup after linearization, before transformation? - (reply: 3)
89. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
90. predict VLP formation - (reply: 1)

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