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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
62. Validating Recombinant Protein - (reply: 1)
63. Sonication of cells in ultrasound bath, without probe - (reply: 3)
64. Proteinase extraction and purification - (reply: 1)
65. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
66. EGFP expression - (reply: 1)
67. the same construct, two stable cell clones with different protein size - (reply: 2)
68. Purification of metallothioneins? - (reply: 1)
69. RIPA buffer for IP and western - (reply: 1)
70. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
71. CO- IP for nuclear protein - (reply: 4)
72. Trichloroacetic Acid precipitation of proteins - (reply: 2)
73. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
74. protein expression with and without induction - (reply: 1)
75. Please help me :Protein expression and purification - (reply: 1)
76. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
77. genotype of e coli strain for protein expression - (reply: 2)
78. Problems with detection of protein expressed in yeast - (reply: 1)
79. thionin - (reply: 1)
80. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
81. secretome and zymograms - (reply: 1)
82. Protein Refolding - Precipitation during dialysis - (reply: 3)
83. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
84. Problem with calculations for enzyme activity - (reply: 2)
85. problem with sds-page - (reply: 8)
86. Ammonium sulfate fractionalization help - (reply: 1)
87. heme protein precipitation - (reply: 4)
88. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
89. regarding protein purification using 8m urea and sds page - (reply: 2)
90. how do you inactivate His-tag affinity beads?? - (reply: 4)

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