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61. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
62. how do GST-tags affect gel filtration results? - (reply: 5)
63. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
64. Stable protein expression in Vero cells - (reply: 2)
65. Which kind of ATP do you usually use in you experiment? - (reply: 2)
66. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
67. GST-tagged protein expression problem! :-( - (reply: 4)
68. Better (quicker) way to pipet glycerol? - (reply: 3)
69. How OD600 influence on IPTG induction and protein expression - (reply: 5)
70. does EGFP dimerize? - (reply: 3)
71. IPTG induction doesn't work:( - (reply: 3)
72. question about transformation - (reply: 1)
73. Mouse IgG1 purification - Protein G - (reply: 1)
74. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
75. what will happen if you let your affinity column beads dry? - (reply: 1)
76. pichia epression problem - (reply: 1)
77. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
78. Multiple protein bands after expression - (reply: 6)
79. his-tag prufication lysis problem:( - (reply: 4)
80. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
81. co-eluted protein with his-tag protein - (reply: 1)
82. Help with His-Tag protein purification - (reply: 2)
83. Discrepancy between tissue and cell culture - (reply: 1)
84. Expression and Lysozyme - (reply: 3)
85. Rosetta-gami B Incubation Time - (reply: 1)
86. Decreased protein yields after successive passaging? - (reply: 1)
87. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
88. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
89. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
90. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
62. how do GST-tags affect gel filtration results? - (reply: 5)
63. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
64. Stable protein expression in Vero cells - (reply: 2)
65. Which kind of ATP do you usually use in you experiment? - (reply: 2)
66. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
67. GST-tagged protein expression problem! :-( - (reply: 4)
68. Better (quicker) way to pipet glycerol? - (reply: 3)
69. How OD600 influence on IPTG induction and protein expression - (reply: 5)
70. does EGFP dimerize? - (reply: 3)
71. IPTG induction doesn't work:( - (reply: 3)
72. question about transformation - (reply: 1)
73. Mouse IgG1 purification - Protein G - (reply: 1)
74. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
75. what will happen if you let your affinity column beads dry? - (reply: 1)
76. pichia epression problem - (reply: 1)
77. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
78. Multiple protein bands after expression - (reply: 6)
79. his-tag prufication lysis problem:( - (reply: 4)
80. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
81. co-eluted protein with his-tag protein - (reply: 1)
82. Help with His-Tag protein purification - (reply: 2)
83. Discrepancy between tissue and cell culture - (reply: 1)
84. Expression and Lysozyme - (reply: 3)
85. Rosetta-gami B Incubation Time - (reply: 1)
86. Decreased protein yields after successive passaging? - (reply: 1)
87. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
88. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
89. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
90. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)