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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. Collagen binding protein expression E. coli His-tag - (reply: 1)
62. pet-32 - (reply: 3)
63. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
64. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
65. Question about MBP purification - (reply: 2)
66. unexpected protein bands - (reply: 2)
67. Urea effect on mice spleen cells - (reply: 1)
68. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
69. Validating Recombinant Protein - (reply: 1)
70. Sonication of cells in ultrasound bath, without probe - (reply: 3)
71. Proteinase extraction and purification - (reply: 1)
72. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
73. EGFP expression - (reply: 1)
74. the same construct, two stable cell clones with different protein size - (reply: 2)
75. Purification of metallothioneins? - (reply: 1)
76. RIPA buffer for IP and western - (reply: 1)
77. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
78. CO- IP for nuclear protein - (reply: 4)
79. Trichloroacetic Acid precipitation of proteins - (reply: 2)
80. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
81. protein expression with and without induction - (reply: 1)
82. Please help me :Protein expression and purification - (reply: 1)
83. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
84. genotype of e coli strain for protein expression - (reply: 2)
85. Problems with detection of protein expressed in yeast - (reply: 1)
86. thionin - (reply: 1)
87. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
88. secretome and zymograms - (reply: 1)
89. Protein Refolding - Precipitation during dialysis - (reply: 3)
90. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)

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