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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. Sonication of cells in ultrasound bath, without probe - (reply: 3)
62. Proteinase extraction and purification - (reply: 1)
63. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
64. EGFP expression - (reply: 1)
65. the same construct, two stable cell clones with different protein size - (reply: 2)
66. Purification of metallothioneins? - (reply: 1)
67. RIPA buffer for IP and western - (reply: 1)
68. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
69. CO- IP for nuclear protein - (reply: 4)
70. Trichloroacetic Acid precipitation of proteins - (reply: 2)
71. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
72. protein expression with and without induction - (reply: 1)
73. Please help me :Protein expression and purification - (reply: 1)
74. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
75. genotype of e coli strain for protein expression - (reply: 2)
76. Problems with detection of protein expressed in yeast - (reply: 1)
77. thionin - (reply: 1)
78. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
79. secretome and zymograms - (reply: 1)
80. Protein Refolding - Precipitation during dialysis - (reply: 3)
81. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
82. Problem with calculations for enzyme activity - (reply: 2)
83. problem with sds-page - (reply: 8)
84. Ammonium sulfate fractionalization help - (reply: 1)
85. heme protein precipitation - (reply: 4)
86. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
87. regarding protein purification using 8m urea and sds page - (reply: 2)
88. how do you inactivate His-tag affinity beads?? - (reply: 4)
89. Protein Purification from mammalian cells - (reply: 1)
90. DNA cleanup after linearization, before transformation? - (reply: 3)

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