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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
62. after protein purification - (reply: 4)
63. Tryple express vs scraping - (reply: 2)
64. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
65. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
66. recombinant protein vs native protein - (reply: 1)
67. protein expression site - (reply: 3)
68. Beads pull down only free GST - (reply: 14)
69. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
70. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
71. protein expression - (reply: 2)
72. Anion Exchange Buffers - (reply: 2)
73. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
74. about sds-page buffers - (reply: 1)
75. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
76. Best epitope for IF with a poor-expresser - (reply: 2)
77. Collagen binding protein expression E. coli His-tag - (reply: 1)
78. pet-32 - (reply: 3)
79. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
80. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
81. Question about MBP purification - (reply: 2)
82. unexpected protein bands - (reply: 2)
83. Urea effect on mice spleen cells - (reply: 1)
84. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
85. Validating Recombinant Protein - (reply: 1)
86. Sonication of cells in ultrasound bath, without probe - (reply: 3)
87. Proteinase extraction and purification - (reply: 1)
88. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
89. EGFP expression - (reply: 1)
90. the same construct, two stable cell clones with different protein size - (reply: 2)

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