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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
61. Beads pull down only free GST - (reply: 14)
62. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
63. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
64. protein expression - (reply: 2)
65. Anion Exchange Buffers - (reply: 2)
66. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
67. about sds-page buffers - (reply: 1)
68. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
69. Best epitope for IF with a poor-expresser - (reply: 2)
70. Collagen binding protein expression E. coli His-tag - (reply: 1)
71. pet-32 - (reply: 3)
72. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
73. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
74. Question about MBP purification - (reply: 2)
75. unexpected protein bands - (reply: 2)
76. Urea effect on mice spleen cells - (reply: 1)
77. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
78. Validating Recombinant Protein - (reply: 1)
79. Sonication of cells in ultrasound bath, without probe - (reply: 3)
80. Proteinase extraction and purification - (reply: 1)
81. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
82. EGFP expression - (reply: 1)
83. the same construct, two stable cell clones with different protein size - (reply: 2)
84. Purification of metallothioneins? - (reply: 1)
85. RIPA buffer for IP and western - (reply: 1)
86. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
87. CO- IP for nuclear protein - (reply: 4)
88. Trichloroacetic Acid precipitation of proteins - (reply: 2)
89. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
90. protein expression with and without induction - (reply: 1)

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