Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
301. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
302. Protein precipitating need help choosing suitable buffering system - (reply: 3)
303. Expressing human protein in E.Coli - (reply: 12)
304. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
305. Help with GST protein purification - (reply: 4)
306. Antiobiotics for protein expression - (reply: 3)
307. how to extract proteins from HEK293 - I need your experience (reply: 3)
308. Identification of purity of the protein - (reply: 2)
309. Polysorbate 80 (Tween 80) removal - (reply: 13)
310. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
311. nuclear extraction of blood cells - (reply: 2)
312. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
313. volume to load sample for affinity chromatography - (reply: 6)
314. Reporter gene to test new promotor - (reply: 1)
315. Protein Phosphorylation - (reply: 2)
316. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
317. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
318. Folding - (reply: 2)
319. C-terminal Cleavage During Overexpression - (reply: 3)
320. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
321. Loss of protein upon concentration - (reply: 4)
322. Problems with Bacterial Induction: NO Induction - (reply: 1)
323. His-tag protein purification - aggregation of protein (reply: 5)
324. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
325. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
326. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
327. Decrease in concentration after protein purification - (reply: 3)
328. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
329. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
330. ways to stop soluble aggregation - (reply: 9)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20