Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Ammonium sulfate fractionalization help - (reply: 1)
92. heme protein precipitation - (reply: 4)
93. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
94. regarding protein purification using 8m urea and sds page - (reply: 2)
95. how do you inactivate His-tag affinity beads?? - (reply: 4)
96. Protein Purification from mammalian cells - (reply: 1)
97. DNA cleanup after linearization, before transformation? - (reply: 3)
98. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
99. predict VLP formation - (reply: 1)
100. need help for transfection to synchronized cells - (reply: 4)
101. Pulldown of polyubiquitinated proteins - (reply: 1)
102. membrane protein expression - (reply: 2)
103. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
104. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
105. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
106. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
107. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
108. protein extraction from cell nucleus - (reply: 2)
109. his-tag purification - (reply: 9)
110. selection of the media for ion exchange chromatography - (reply: 1)
111. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
112. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
113. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
114. His-tag and GST-tag - (reply: 1)
115. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
116. Do protein purification under partially denatured condition - (reply: 1)
117. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
118. how to remove affinity tag completely - (reply: 14)
119. Agarose bead size in affinity chromatography - (reply: 2)
120. Protein precipitation by storage - (reply: 11)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22