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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. need help for transfection to synchronized cells - (reply: 4)
92. Pulldown of polyubiquitinated proteins - (reply: 1)
93. membrane protein expression - (reply: 2)
94. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
95. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
96. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
97. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
98. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
99. protein extraction from cell nucleus - (reply: 2)
100. his-tag purification - (reply: 9)
101. selection of the media for ion exchange chromatography - (reply: 1)
102. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
103. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
104. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
105. His-tag and GST-tag - (reply: 1)
106. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
107. Do protein purification under partially denatured condition - (reply: 1)
108. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
109. how to remove affinity tag completely - (reply: 14)
110. Agarose bead size in affinity chromatography - (reply: 2)
111. Protein precipitation by storage - (reply: 11)
112. designing fusion protein with Flag tag - (reply: 1)
113. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
114. Control protein for eukaryotic expression - (reply: 5)
115. Flag fusion protein not detected by Flag antibody - (reply: 8)
116. E. coli culture - (reply: 1)
117. instability index of expressed protein - (reply: 1)
118. Protein Purification-catastrophe while purifying? - (reply: 2)
119. T5 Promoter - (reply: 1)
120. Protein purification for adherent mammalian cells - (reply: 2)

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