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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. genotype of e coli strain for protein expression - (reply: 2)
92. Problems with detection of protein expressed in yeast - (reply: 1)
93. thionin - (reply: 1)
94. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
95. secretome and zymograms - (reply: 1)
96. Protein Refolding - Precipitation during dialysis - (reply: 3)
97. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
98. Problem with calculations for enzyme activity - (reply: 2)
99. problem with sds-page - (reply: 8)
100. Ammonium sulfate fractionalization help - (reply: 1)
101. heme protein precipitation - (reply: 4)
102. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
103. regarding protein purification using 8m urea and sds page - (reply: 2)
104. how do you inactivate His-tag affinity beads?? - (reply: 4)
105. Protein Purification from mammalian cells - (reply: 1)
106. DNA cleanup after linearization, before transformation? - (reply: 3)
107. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
108. predict VLP formation - (reply: 1)
109. need help for transfection to synchronized cells - (reply: 4)
110. Pulldown of polyubiquitinated proteins - (reply: 1)
111. membrane protein expression - (reply: 2)
112. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
113. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
114. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
115. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
116. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
117. protein extraction from cell nucleus - (reply: 2)
118. his-tag purification - (reply: 9)
119. selection of the media for ion exchange chromatography - (reply: 1)
120. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)

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