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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. DNA cleanup after linearization, before transformation? - (reply: 3)
92. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
93. predict VLP formation - (reply: 1)
94. need help for transfection to synchronized cells - (reply: 4)
95. Pulldown of polyubiquitinated proteins - (reply: 1)
96. membrane protein expression - (reply: 2)
97. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
98. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
99. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
100. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
101. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
102. protein extraction from cell nucleus - (reply: 2)
103. his-tag purification - (reply: 9)
104. selection of the media for ion exchange chromatography - (reply: 1)
105. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
106. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
107. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
108. His-tag and GST-tag - (reply: 1)
109. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
110. Do protein purification under partially denatured condition - (reply: 1)
111. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
112. how to remove affinity tag completely - (reply: 14)
113. Agarose bead size in affinity chromatography - (reply: 2)
114. Protein precipitation by storage - (reply: 11)
115. designing fusion protein with Flag tag - (reply: 1)
116. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
117. Control protein for eukaryotic expression - (reply: 5)
118. Flag fusion protein not detected by Flag antibody - (reply: 8)
119. E. coli culture - (reply: 1)
120. instability index of expressed protein - (reply: 1)

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