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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
92. regarding protein purification using 8m urea and sds page - (reply: 2)
93. how do you inactivate His-tag affinity beads?? - (reply: 4)
94. Protein Purification from mammalian cells - (reply: 1)
95. DNA cleanup after linearization, before transformation? - (reply: 3)
96. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
97. predict VLP formation - (reply: 1)
98. need help for transfection to synchronized cells - (reply: 4)
99. Pulldown of polyubiquitinated proteins - (reply: 1)
100. membrane protein expression - (reply: 2)
101. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
102. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
103. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
104. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
105. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
106. protein extraction from cell nucleus - (reply: 2)
107. his-tag purification - (reply: 9)
108. selection of the media for ion exchange chromatography - (reply: 1)
109. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
110. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
111. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
112. His-tag and GST-tag - (reply: 1)
113. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
114. Do protein purification under partially denatured condition - (reply: 1)
115. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
116. how to remove affinity tag completely - (reply: 14)
117. Agarose bead size in affinity chromatography - (reply: 2)
118. Protein precipitation by storage - (reply: 11)
119. designing fusion protein with Flag tag - (reply: 1)
120. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)

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