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91. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
92. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
93. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
94. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
95. how much time for prepared gel to keep bfore loading? - (reply: 5)
96. plant extraction procedure for protein - (reply: 3)
97. brain tissue homogenization with sample buffer?? - (reply: 2)
98. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
99. Reversed-Phase chromatography for protein purification - (reply: 2)
100. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
101. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
102. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
103. need help with gst-tagged protein-purification - (reply: 1)
104. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
105. Protein degradation during expression - (reply: 3)
106. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
107. Expression of insoluble protein - (reply: 4)
108. Weak affinity chromatography - (reply: 2)
109. NL and L? - (reply: 2)
110. e coli vector and host selection - (reply: 1)
111. Double tagging - (reply: 1)
112. Purifying recombinant protein from pellet? - (reply: 2)
113. Co-expression of 2 plasmids in E.coli - (reply: 1)
114. Protein ladder - (reply: 1)
115. Trouble producing stable cell lines - (reply: 2)
116. Can anyone help me to identify what line is that??? - (reply: 4)
117. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
118. membrane protein expression - (reply: 1)
119. Magnesium acetate in protein lysates - (reply: 1)
120. Protease Inhibitor Cocktails - (reply: 3)
92. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
93. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
94. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
95. how much time for prepared gel to keep bfore loading? - (reply: 5)
96. plant extraction procedure for protein - (reply: 3)
97. brain tissue homogenization with sample buffer?? - (reply: 2)
98. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
99. Reversed-Phase chromatography for protein purification - (reply: 2)
100. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
101. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
102. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
103. need help with gst-tagged protein-purification - (reply: 1)
104. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
105. Protein degradation during expression - (reply: 3)
106. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
107. Expression of insoluble protein - (reply: 4)
108. Weak affinity chromatography - (reply: 2)
109. NL and L? - (reply: 2)
110. e coli vector and host selection - (reply: 1)
111. Double tagging - (reply: 1)
112. Purifying recombinant protein from pellet? - (reply: 2)
113. Co-expression of 2 plasmids in E.coli - (reply: 1)
114. Protein ladder - (reply: 1)
115. Trouble producing stable cell lines - (reply: 2)
116. Can anyone help me to identify what line is that??? - (reply: 4)
117. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
118. membrane protein expression - (reply: 1)
119. Magnesium acetate in protein lysates - (reply: 1)
120. Protease Inhibitor Cocktails - (reply: 3)