Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
92. genotype of e coli strain for protein expression - (reply: 2)
93. Problems with detection of protein expressed in yeast - (reply: 1)
94. thionin - (reply: 1)
95. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
96. secretome and zymograms - (reply: 1)
97. Protein Refolding - Precipitation during dialysis - (reply: 3)
98. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
99. Problem with calculations for enzyme activity - (reply: 2)
100. problem with sds-page - (reply: 8)
101. Ammonium sulfate fractionalization help - (reply: 1)
102. heme protein precipitation - (reply: 4)
103. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
104. regarding protein purification using 8m urea and sds page - (reply: 2)
105. how do you inactivate His-tag affinity beads?? - (reply: 4)
106. Protein Purification from mammalian cells - (reply: 1)
107. DNA cleanup after linearization, before transformation? - (reply: 3)
108. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
109. predict VLP formation - (reply: 1)
110. need help for transfection to synchronized cells - (reply: 4)
111. Pulldown of polyubiquitinated proteins - (reply: 1)
112. membrane protein expression - (reply: 2)
113. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
114. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
115. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
116. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
117. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
118. protein extraction from cell nucleus - (reply: 2)
119. his-tag purification - (reply: 9)
120. selection of the media for ion exchange chromatography - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22