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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. how do you inactivate His-tag affinity beads?? - (reply: 4)
92. Protein Purification from mammalian cells - (reply: 1)
93. DNA cleanup after linearization, before transformation? - (reply: 3)
94. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
95. predict VLP formation - (reply: 1)
96. need help for transfection to synchronized cells - (reply: 4)
97. Pulldown of polyubiquitinated proteins - (reply: 1)
98. membrane protein expression - (reply: 2)
99. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
100. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
101. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
102. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
103. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
104. protein extraction from cell nucleus - (reply: 2)
105. his-tag purification - (reply: 9)
106. selection of the media for ion exchange chromatography - (reply: 1)
107. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
108. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
109. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
110. His-tag and GST-tag - (reply: 1)
111. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
112. Do protein purification under partially denatured condition - (reply: 1)
113. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
114. how to remove affinity tag completely - (reply: 14)
115. Agarose bead size in affinity chromatography - (reply: 2)
116. Protein precipitation by storage - (reply: 11)
117. designing fusion protein with Flag tag - (reply: 1)
118. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
119. Control protein for eukaryotic expression - (reply: 5)
120. Flag fusion protein not detected by Flag antibody - (reply: 8)

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