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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
92. Problem with calculations for enzyme activity - (reply: 2)
93. problem with sds-page - (reply: 8)
94. Ammonium sulfate fractionalization help - (reply: 1)
95. heme protein precipitation - (reply: 4)
96. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
97. regarding protein purification using 8m urea and sds page - (reply: 2)
98. how do you inactivate His-tag affinity beads?? - (reply: 4)
99. Protein Purification from mammalian cells - (reply: 1)
100. DNA cleanup after linearization, before transformation? - (reply: 3)
101. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
102. predict VLP formation - (reply: 1)
103. need help for transfection to synchronized cells - (reply: 4)
104. Pulldown of polyubiquitinated proteins - (reply: 1)
105. membrane protein expression - (reply: 2)
106. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
107. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
108. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
109. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
110. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
111. protein extraction from cell nucleus - (reply: 2)
112. his-tag purification - (reply: 9)
113. selection of the media for ion exchange chromatography - (reply: 1)
114. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
115. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
116. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
117. His-tag and GST-tag - (reply: 1)
118. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
119. Do protein purification under partially denatured condition - (reply: 1)
120. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)

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