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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Protein purifcation without chromatography - (reply: 6)
92. Bacterial Media Formulation - (reply: 2)
93. tag for protein purification - (reply: 7)
94. Protein is not Binding to DE52 column material - (reply: 2)
95. expression problem with pBAD33...similar experience? - (reply: 2)
96. MBP-Tagged protein degrading after expression - (reply: 1)
97. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
98. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
99. Controls for co-immunoprecipitation - (reply: 3)
100. How to improve enzyme activity? - (reply: 2)
101. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
102. Sediments/debris/pellet in protein lysate? - (reply: 3)
103. Cleavage of GST-tag with Factor Xa - (reply: 1)
104. Trouble in GST- fusion protein purification - (reply: 1)
105. Helppurification of expressed protein - (reply: 2)
106. protein expression by changing start codon - (reply: 1)
107. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
108. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
109. pcDNA overexpression - (reply: 4)
110. membrane proteins ultrafiltration - (reply: 2)
111. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
112. Interferon Alpha - (reply: 1)
113. Can you see GFP after lysis mammalian cells? - (reply: 5)
114. Very small peptide expression in E. coli - (reply: 2)
115. C-terminal GST fusion problem in mammalian cell - (reply: 6)
116. Is anti-His antibody detergent sensitive? - (reply: 6)
117. tagged protein size - (reply: 10)
118. Protein expression in e.coli - (reply: 5)
119. Problems with overnights - (reply: 5)
120. problem in extracting the protein - (reply: 1)

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