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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Contaminants in protein purification.....help! - (reply: 3)
92. Protein expression and purification - Please help - (reply: 2)
93. Protein purifcation without chromatography - (reply: 6)
94. Bacterial Media Formulation - (reply: 2)
95. tag for protein purification - (reply: 7)
96. Protein is not Binding to DE52 column material - (reply: 2)
97. expression problem with pBAD33...similar experience? - (reply: 2)
98. MBP-Tagged protein degrading after expression - (reply: 1)
99. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
100. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
101. Controls for co-immunoprecipitation - (reply: 3)
102. How to improve enzyme activity? - (reply: 2)
103. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
104. Sediments/debris/pellet in protein lysate? - (reply: 3)
105. Cleavage of GST-tag with Factor Xa - (reply: 1)
106. Trouble in GST- fusion protein purification - (reply: 1)
107. Helppurification of expressed protein - (reply: 2)
108. protein expression by changing start codon - (reply: 1)
109. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
110. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
111. pcDNA overexpression - (reply: 4)
112. membrane proteins ultrafiltration - (reply: 2)
113. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
114. Interferon Alpha - (reply: 1)
115. Can you see GFP after lysis mammalian cells? - (reply: 5)
116. Very small peptide expression in E. coli - (reply: 2)
117. C-terminal GST fusion problem in mammalian cell - (reply: 6)
118. Is anti-His antibody detergent sensitive? - (reply: 6)
119. tagged protein size - (reply: 10)
120. Protein expression in e.coli - (reply: 5)

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