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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
91. Validating Recombinant Protein - (reply: 1)
92. Sonication of cells in ultrasound bath, without probe - (reply: 3)
93. Proteinase extraction and purification - (reply: 1)
94. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
95. EGFP expression - (reply: 1)
96. the same construct, two stable cell clones with different protein size - (reply: 2)
97. Purification of metallothioneins? - (reply: 1)
98. RIPA buffer for IP and western - (reply: 1)
99. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
100. CO- IP for nuclear protein - (reply: 4)
101. Trichloroacetic Acid precipitation of proteins - (reply: 2)
102. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
103. protein expression with and without induction - (reply: 1)
104. Please help me :Protein expression and purification - (reply: 1)
105. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
106. genotype of e coli strain for protein expression - (reply: 2)
107. Problems with detection of protein expressed in yeast - (reply: 1)
108. thionin - (reply: 1)
109. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
110. secretome and zymograms - (reply: 1)
111. Protein Refolding - Precipitation during dialysis - (reply: 3)
112. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
113. Problem with calculations for enzyme activity - (reply: 2)
114. problem with sds-page - (reply: 8)
115. Ammonium sulfate fractionalization help - (reply: 1)
116. heme protein precipitation - (reply: 4)
117. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
118. regarding protein purification using 8m urea and sds page - (reply: 2)
119. how do you inactivate His-tag affinity beads?? - (reply: 4)
120. Protein Purification from mammalian cells - (reply: 1)

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