GST tag fusion protein--- help please - (Jul/14/2013 )
Is the protein at the approriate MW on a western? Perhaps the GST fusion is impeding enzymatic activity? If this is the case, there are several cleavable linkers you could use.
Good Luck! GST often dimerizes taking your target protein with it. This can interfere with functionality. Cleaving seems like a great idea.
I have tried several times to purify the protein,and I it`s really a hard work .Could you give me some advise about using thrombin to cleavage the FUSION？