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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
392. Folding - (reply: 2)
393. C-terminal Cleavage During Overexpression - (reply: 3)
394. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
395. Loss of protein upon concentration - (reply: 4)
396. Problems with Bacterial Induction: NO Induction - (reply: 1)
397. His-tag protein purification - aggregation of protein (reply: 5)
398. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
399. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
400. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
401. Decrease in concentration after protein purification - (reply: 3)
402. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
403. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
404. ways to stop soluble aggregation - (reply: 9)
405. need protocol on purfication of allinase from raw garlic - (reply: 2)
406. labeling a protein - (reply: 3)
407. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
408. How to be good at prep? - Calling all protein experts (reply: 2)
409. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
410. His-tagged protein purification - (reply: 3)
411. protein purification - (reply: 3)
412. pGEX vectors and GST expression - (reply: 3)
413. contamination in protein expression - I got a couple of questions (reply: 3)
414. Always expressing truncated protein - (reply: 3)
415. Over Experssion of A Protein - (reply: 3)
416. IPTG induction not observable or noticeable - (reply: 2)
417. Problem with protein purification By FPLC - (reply: 4)
418. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
419. His-tag proteins not eluted from Ni-Beads - (reply: 2)
420. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)

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