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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. His-tag protein purification - aggregation of protein (reply: 5)
392. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
393. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
394. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
395. Decrease in concentration after protein purification - (reply: 3)
396. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
397. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
398. ways to stop soluble aggregation - (reply: 9)
399. need protocol on purfication of allinase from raw garlic - (reply: 2)
400. labeling a protein - (reply: 3)
401. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
402. How to be good at prep? - Calling all protein experts (reply: 2)
403. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
404. His-tagged protein purification - (reply: 3)
405. protein purification - (reply: 3)
406. pGEX vectors and GST expression - (reply: 3)
407. contamination in protein expression - I got a couple of questions (reply: 3)
408. Always expressing truncated protein - (reply: 3)
409. Over Experssion of A Protein - (reply: 3)
410. IPTG induction not observable or noticeable - (reply: 2)
411. Problem with protein purification By FPLC - (reply: 4)
412. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
413. His-tag proteins not eluted from Ni-Beads - (reply: 2)
414. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
415. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
416. trouble with protein expression of high AT rich gene - (reply: 1)
417. Preparation of Purified Inclusion Bodies - (reply: 2)
418. Denaturing IB in 8 M Urea - (reply: 3)
419. Promega IVT Problems - Low expression (reply: 1)
420. weak bands - western blots (reply: 1)

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