Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. Protein Phosphorylation - (reply: 2)
392. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
393. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
394. Folding - (reply: 2)
395. C-terminal Cleavage During Overexpression - (reply: 3)
396. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
397. Loss of protein upon concentration - (reply: 4)
398. Problems with Bacterial Induction: NO Induction - (reply: 1)
399. His-tag protein purification - aggregation of protein (reply: 5)
400. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
401. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
402. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
403. Decrease in concentration after protein purification - (reply: 3)
404. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
405. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
406. ways to stop soluble aggregation - (reply: 9)
407. need protocol on purfication of allinase from raw garlic - (reply: 2)
408. labeling a protein - (reply: 3)
409. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
410. How to be good at prep? - Calling all protein experts (reply: 2)
411. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
412. His-tagged protein purification - (reply: 3)
413. protein purification - (reply: 3)
414. pGEX vectors and GST expression - (reply: 3)
415. contamination in protein expression - I got a couple of questions (reply: 3)
416. Always expressing truncated protein - (reply: 3)
417. Over Experssion of A Protein - (reply: 3)
418. IPTG induction not observable or noticeable - (reply: 2)
419. Problem with protein purification By FPLC - (reply: 4)
420. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22