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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. Problem with MBP and GST tag purification - (reply: 1)
392. concentrating protein by method other than tff - (reply: 1)
393. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
394. M2 Flag purification - (reply: 2)
395. Quantitating IP'd proteins - (reply: 1)
396. Removal of Tightly Bound Nucleic Acids - (reply: 3)
397. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
398. Recombinant protein aggregation after purification - (reply: 2)
399. average total protein content per 293t cell - (reply: 2)
400. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
401. expression of MBP fusion protein - leaky and very low yield (reply: 2)
402. Calmodulin binding assay - (reply: 1)
403. strange problem with His purification - (reply: 1)
404. E.coli expression - (reply: 5)
405. why my protein precipitates? - (reply: 2)
406. DTT in GST elution buffer - (reply: 1)
407. vectors and his-tags - (reply: 3)
408. SDS boiled samples - (reply: 1)
409. E coli lysis by lysozyme - (reply: 2)
410. His-tag protein expression - (reply: 1)
411. SDS-PAGE loading dye - (reply: 1)
412. How to concentrate the protein - (reply: 4)
413. protein overexpression - factors affecting overexpression (reply: 3)
414. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
415. e coli strain selection for protein expression - (reply: 2)
416. Problem clariflying/filtering insect cell lysate - (reply: 2)
417. Ni column changing colors - (reply: 2)
418. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
419. Problem with Histag purification - (reply: 1)
420. Freezing eluted His tag protein - (reply: 5)

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