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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. Polysorbate 80 (Tween 80) removal - (reply: 13)
392. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
393. nuclear extraction of blood cells - (reply: 2)
394. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
395. volume to load sample for affinity chromatography - (reply: 6)
396. Reporter gene to test new promotor - (reply: 1)
397. Protein Phosphorylation - (reply: 2)
398. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
399. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
400. Folding - (reply: 2)
401. C-terminal Cleavage During Overexpression - (reply: 3)
402. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
403. Loss of protein upon concentration - (reply: 4)
404. Problems with Bacterial Induction: NO Induction - (reply: 1)
405. His-tag protein purification - aggregation of protein (reply: 5)
406. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
407. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
408. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
409. Decrease in concentration after protein purification - (reply: 3)
410. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
411. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
412. ways to stop soluble aggregation - (reply: 9)
413. need protocol on purfication of allinase from raw garlic - (reply: 2)
414. labeling a protein - (reply: 3)
415. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
416. How to be good at prep? - Calling all protein experts (reply: 2)
417. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
418. His-tagged protein purification - (reply: 3)
419. protein purification - (reply: 3)
420. pGEX vectors and GST expression - (reply: 3)

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