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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
392. How to be good at prep? - Calling all protein experts (reply: 2)
393. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
394. His-tagged protein purification - (reply: 3)
395. protein purification - (reply: 3)
396. pGEX vectors and GST expression - (reply: 3)
397. contamination in protein expression - I got a couple of questions (reply: 3)
398. Always expressing truncated protein - (reply: 3)
399. Over Experssion of A Protein - (reply: 3)
400. IPTG induction not observable or noticeable - (reply: 2)
401. Problem with protein purification By FPLC - (reply: 4)
402. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
403. His-tag proteins not eluted from Ni-Beads - (reply: 2)
404. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
405. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
406. trouble with protein expression of high AT rich gene - (reply: 1)
407. Preparation of Purified Inclusion Bodies - (reply: 2)
408. Denaturing IB in 8 M Urea - (reply: 3)
409. Promega IVT Problems - Low expression (reply: 1)
410. weak bands - western blots (reply: 1)
411. problem in M9 media - m9 media (reply: 4)
412. Help for a purificaltion newbie - (reply: 2)
413. Immunioprecipitation from Jurkat cells?? - (reply: 1)
414. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
415. antibody HRP - (reply: 2)
416. Western blot transfer weak - (reply: 1)
417. Home-made Size-exclusion Columns? - (reply: 1)
418. how to get pure protein - (reply: 1)
419. co-Immunoprecipitation control question - co-IP (reply: 1)
420. to get protein in cytosol from inclusion bodies - (reply: 4)

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