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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. Protein Purification by precipitation - (reply: 2)
392. refolding proteins using dialysis - (reply: 1)
393. protein refolding - (reply: 1)
394. Zeocine resistence, pichia pastoris - (reply: 3)
395. Problem with MBP and GST tag purification - (reply: 1)
396. concentrating protein by method other than tff - (reply: 1)
397. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
398. M2 Flag purification - (reply: 2)
399. Quantitating IP'd proteins - (reply: 1)
400. Removal of Tightly Bound Nucleic Acids - (reply: 3)
401. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
402. Recombinant protein aggregation after purification - (reply: 2)
403. average total protein content per 293t cell - (reply: 2)
404. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
405. expression of MBP fusion protein - leaky and very low yield (reply: 2)
406. Calmodulin binding assay - (reply: 1)
407. strange problem with His purification - (reply: 1)
408. E.coli expression - (reply: 5)
409. why my protein precipitates? - (reply: 2)
410. DTT in GST elution buffer - (reply: 1)
411. vectors and his-tags - (reply: 3)
412. SDS boiled samples - (reply: 1)
413. E coli lysis by lysozyme - (reply: 2)
414. His-tag protein expression - (reply: 1)
415. SDS-PAGE loading dye - (reply: 1)
416. How to concentrate the protein - (reply: 4)
417. protein overexpression - factors affecting overexpression (reply: 3)
418. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
419. e coli strain selection for protein expression - (reply: 2)
420. Problem clariflying/filtering insect cell lysate - (reply: 2)

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