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391. Can GST-tag be separated from GST-fusion protein - (reply: 1)
392. Protein analog - (reply: 1)
393. membrane protein extraction buffer - (reply: 1)
394. What about the Baculovirus expression system? - (reply: 2)
395. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
396. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
397. protein is degraded after purification - (reply: 2)
398. protein expression - Preparation of starter cultures (reply: 15)
399. Problem with Protein elution using GST sepharose 4B - (reply: 1)
400. how to isolate insoluble protein in bacterial induction? - (reply: 4)
401. Plasmid instability in E.coli BL21? - (reply: 3)
402. Cleaving Fusion Proteins - (reply: 1)
403. Plyse cell and DH5a - (reply: 1)
404. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
405. Electroelution with no electroeluter - (reply: 3)
406. reusing Ni NTA - (reply: 2)
407. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
408. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
409. Checking For Protein Expression - (reply: 3)
410. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
411. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
412. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
413. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
414. Couple questions about GST purification - (reply: 16)
415. Immunoprecipitation - (reply: 12)
416. Newbie question: how to determine protein concentration after IP? - (reply: 8)
417. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
418. High pressure on Ni-NTA beads - (reply: 2)
419. affinity column problems - (reply: 1)
420. Desalting protein - (reply: 7)
392. Protein analog - (reply: 1)
393. membrane protein extraction buffer - (reply: 1)
394. What about the Baculovirus expression system? - (reply: 2)
395. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
396. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
397. protein is degraded after purification - (reply: 2)
398. protein expression - Preparation of starter cultures (reply: 15)
399. Problem with Protein elution using GST sepharose 4B - (reply: 1)
400. how to isolate insoluble protein in bacterial induction? - (reply: 4)
401. Plasmid instability in E.coli BL21? - (reply: 3)
402. Cleaving Fusion Proteins - (reply: 1)
403. Plyse cell and DH5a - (reply: 1)
404. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
405. Electroelution with no electroeluter - (reply: 3)
406. reusing Ni NTA - (reply: 2)
407. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
408. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
409. Checking For Protein Expression - (reply: 3)
410. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
411. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
412. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
413. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
414. Couple questions about GST purification - (reply: 16)
415. Immunoprecipitation - (reply: 12)
416. Newbie question: how to determine protein concentration after IP? - (reply: 8)
417. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
418. High pressure on Ni-NTA beads - (reply: 2)
419. affinity column problems - (reply: 1)
420. Desalting protein - (reply: 7)