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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
391. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
392. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
393. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
394. Decrease in concentration after protein purification - (reply: 3)
395. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
396. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
397. ways to stop soluble aggregation - (reply: 9)
398. need protocol on purfication of allinase from raw garlic - (reply: 2)
399. labeling a protein - (reply: 3)
400. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
401. How to be good at prep? - Calling all protein experts (reply: 2)
402. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
403. His-tagged protein purification - (reply: 3)
404. protein purification - (reply: 3)
405. pGEX vectors and GST expression - (reply: 3)
406. contamination in protein expression - I got a couple of questions (reply: 3)
407. Always expressing truncated protein - (reply: 3)
408. Over Experssion of A Protein - (reply: 3)
409. IPTG induction not observable or noticeable - (reply: 2)
410. Problem with protein purification By FPLC - (reply: 4)
411. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
412. His-tag proteins not eluted from Ni-Beads - (reply: 2)
413. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
414. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
415. trouble with protein expression of high AT rich gene - (reply: 1)
416. Preparation of Purified Inclusion Bodies - (reply: 2)
417. Denaturing IB in 8 M Urea - (reply: 3)
418. Promega IVT Problems - Low expression (reply: 1)
419. weak bands - western blots (reply: 1)
420. problem in M9 media - m9 media (reply: 4)

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