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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
242. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
243. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
244. need help with gst-tagged protein-purification - (reply: 1)
245. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
246. Protein degradation during expression - (reply: 3)
247. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
248. Expression of insoluble protein - (reply: 4)
249. Weak affinity chromatography - (reply: 2)
250. NL and L? - (reply: 2)
251. e coli vector and host selection - (reply: 1)
252. Double tagging - (reply: 1)
253. Purifying recombinant protein from pellet? - (reply: 2)
254. Co-expression of 2 plasmids in E.coli - (reply: 1)
255. Protein ladder - (reply: 1)
256. Trouble producing stable cell lines - (reply: 2)
257. Can anyone help me to identify what line is that??? - (reply: 4)
258. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
259. membrane protein expression - (reply: 1)
260. Magnesium acetate in protein lysates - (reply: 1)
261. Protease Inhibitor Cocktails - (reply: 3)
262. E. coli cells refuse to grow! - (reply: 2)
263. Extra bands after protein expression/purification - (reply: 4)
264. Better protein Expression? - (reply: 2)
265. Expression of protein with pI 8.2 - (reply: 1)
266. Methionine for protein expression - (reply: 3)
267. Alternatives to benzonase? - (reply: 1)
268. Protein Expression problems - (reply: 4)
269. Advice on soluble aggregate problem - (reply: 2)
270. problem in expressing A DNA binding protein - (reply: 6)

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