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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
242. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
243. how much time for prepared gel to keep bfore loading? - (reply: 5)
244. plant extraction procedure for protein - (reply: 3)
245. brain tissue homogenization with sample buffer?? - (reply: 2)
246. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
247. Reversed-Phase chromatography for protein purification - (reply: 3)
248. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
249. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
250. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
251. need help with gst-tagged protein-purification - (reply: 1)
252. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
253. Protein degradation during expression - (reply: 3)
254. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
255. Expression of insoluble protein - (reply: 4)
256. Weak affinity chromatography - (reply: 2)
257. NL and L? - (reply: 2)
258. e coli vector and host selection - (reply: 1)
259. Double tagging - (reply: 1)
260. Purifying recombinant protein from pellet? - (reply: 2)
261. Co-expression of 2 plasmids in E.coli - (reply: 1)
262. Protein ladder - (reply: 1)
263. Trouble producing stable cell lines - (reply: 2)
264. Can anyone help me to identify what line is that??? - (reply: 4)
265. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
266. membrane protein expression - (reply: 1)
267. Magnesium acetate in protein lysates - (reply: 1)
268. Protease Inhibitor Cocktails - (reply: 3)
269. E. coli cells refuse to grow! - (reply: 2)
270. Extra bands after protein expression/purification - (reply: 4)

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