Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
242. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
243. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
244. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
245. how much time for prepared gel to keep bfore loading? - (reply: 5)
246. plant extraction procedure for protein - (reply: 3)
247. brain tissue homogenization with sample buffer?? - (reply: 2)
248. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
249. Reversed-Phase chromatography for protein purification - (reply: 3)
250. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
251. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
252. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
253. need help with gst-tagged protein-purification - (reply: 1)
254. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
255. Protein degradation during expression - (reply: 3)
256. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
257. Expression of insoluble protein - (reply: 4)
258. Weak affinity chromatography - (reply: 2)
259. NL and L? - (reply: 2)
260. e coli vector and host selection - (reply: 1)
261. Double tagging - (reply: 1)
262. Purifying recombinant protein from pellet? - (reply: 2)
263. Co-expression of 2 plasmids in E.coli - (reply: 1)
264. Protein ladder - (reply: 1)
265. Trouble producing stable cell lines - (reply: 2)
266. Can anyone help me to identify what line is that??? - (reply: 4)
267. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
268. membrane protein expression - (reply: 1)
269. Magnesium acetate in protein lysates - (reply: 1)
270. Protease Inhibitor Cocktails - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22