Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. pichia epression problem - (reply: 1)
242. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
243. Multiple protein bands after expression - (reply: 6)
244. his-tag prufication lysis problem:( - (reply: 4)
245. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
246. co-eluted protein with his-tag protein - (reply: 1)
247. Help with His-Tag protein purification - (reply: 2)
248. Discrepancy between tissue and cell culture - (reply: 1)
249. Expression and Lysozyme - (reply: 3)
250. Rosetta-gami B Incubation Time - (reply: 1)
251. Decreased protein yields after successive passaging? - (reply: 1)
252. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
253. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
254. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
255. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
256. how much time for prepared gel to keep bfore loading? - (reply: 5)
257. plant extraction procedure for protein - (reply: 3)
258. brain tissue homogenization with sample buffer?? - (reply: 2)
259. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
260. Reversed-Phase chromatography for protein purification - (reply: 3)
261. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
262. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
263. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
264. need help with gst-tagged protein-purification - (reply: 1)
265. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
266. Protein degradation during expression - (reply: 3)
267. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
268. Expression of insoluble protein - (reply: 4)
269. Weak affinity chromatography - (reply: 2)
270. NL and L? - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22