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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
242. what will happen if you let your affinity column beads dry? - (reply: 1)
243. pichia epression problem - (reply: 1)
244. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
245. Multiple protein bands after expression - (reply: 6)
246. his-tag prufication lysis problem:( - (reply: 4)
247. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
248. co-eluted protein with his-tag protein - (reply: 1)
249. Help with His-Tag protein purification - (reply: 2)
250. Discrepancy between tissue and cell culture - (reply: 1)
251. Expression and Lysozyme - (reply: 3)
252. Rosetta-gami B Incubation Time - (reply: 1)
253. Decreased protein yields after successive passaging? - (reply: 1)
254. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
255. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
256. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
257. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
258. how much time for prepared gel to keep bfore loading? - (reply: 5)
259. plant extraction procedure for protein - (reply: 3)
260. brain tissue homogenization with sample buffer?? - (reply: 2)
261. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
262. Reversed-Phase chromatography for protein purification - (reply: 3)
263. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
264. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
265. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
266. need help with gst-tagged protein-purification - (reply: 1)
267. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
268. Protein degradation during expression - (reply: 3)
269. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
270. Expression of insoluble protein - (reply: 4)

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