Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. what will happen if you let your affinity column beads dry? - (reply: 1)
242. pichia epression problem - (reply: 1)
243. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
244. Multiple protein bands after expression - (reply: 6)
245. his-tag prufication lysis problem:( - (reply: 4)
246. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
247. co-eluted protein with his-tag protein - (reply: 1)
248. Help with His-Tag protein purification - (reply: 2)
249. Discrepancy between tissue and cell culture - (reply: 1)
250. Expression and Lysozyme - (reply: 3)
251. Rosetta-gami B Incubation Time - (reply: 1)
252. Decreased protein yields after successive passaging? - (reply: 1)
253. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
254. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
255. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
256. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
257. how much time for prepared gel to keep bfore loading? - (reply: 5)
258. plant extraction procedure for protein - (reply: 3)
259. brain tissue homogenization with sample buffer?? - (reply: 2)
260. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
261. Reversed-Phase chromatography for protein purification - (reply: 3)
262. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
263. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
264. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
265. need help with gst-tagged protein-purification - (reply: 1)
266. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
267. Protein degradation during expression - (reply: 3)
268. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
269. Expression of insoluble protein - (reply: 4)
270. Weak affinity chromatography - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22