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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
241. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
242. GST-tagged protein expression problem! :-( - (reply: 4)
243. Better (quicker) way to pipet glycerol? - (reply: 3)
244. How OD600 influence on IPTG induction and protein expression - (reply: 5)
245. does EGFP dimerize? - (reply: 3)
246. IPTG induction doesn't work:( - (reply: 3)
247. question about transformation - (reply: 1)
248. Mouse IgG1 purification - Protein G - (reply: 1)
249. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
250. what will happen if you let your affinity column beads dry? - (reply: 1)
251. pichia epression problem - (reply: 1)
252. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
253. Multiple protein bands after expression - (reply: 6)
254. his-tag prufication lysis problem:( - (reply: 4)
255. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
256. co-eluted protein with his-tag protein - (reply: 1)
257. Help with His-Tag protein purification - (reply: 2)
258. Discrepancy between tissue and cell culture - (reply: 1)
259. Expression and Lysozyme - (reply: 3)
260. Rosetta-gami B Incubation Time - (reply: 1)
261. Decreased protein yields after successive passaging? - (reply: 1)
262. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
263. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
264. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
265. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
266. how much time for prepared gel to keep bfore loading? - (reply: 5)
267. plant extraction procedure for protein - (reply: 3)
268. brain tissue homogenization with sample buffer?? - (reply: 2)
269. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
270. Reversed-Phase chromatography for protein purification - (reply: 3)

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